Objective To explore the effect and mechanism of gamma-tocopherol(γ-T) in asthmatic mice. Methods A total of 40 male Balb/c mice(4weeks,18~20 g) were randomly divided into four groups:asthma group,dexamethasone group,γ-T group,and normal group.The mice of asthma group were received intraperitoneal injection of OVA (10 μg) on day 0,7,14 and exposed to 25 g/L OVA inhalation from day 21 for one week.Ovalbumin (OVA)-sensitized mice were challenged with dexamethasone and γ-T for one week,the normal group were only given saline.Blood plasma and BALF samples were collected after the 28 d mice were sacrificed.The concentration of eotaxin in serum and BALF were determined by the method of ELISA.One-way ANOVA was used test the difference between groups. Results The concentration of eotaxin in serum and BALF of asthmatic mice were higher than normal,and those were lower in dexamethasone and γ-T group.The concentration of eotaxin in serum of γ-T group were lower compared with asthma and dexamethasone group(P>0.05);The concentration of eotaxin in BALF of γ-T group were lower compared with dexamethasone and asthma group(P all<0.05). Conclusion γ-T prohibit the expression of eotaxin in asthmatic mice,and γ-T might be an effective therapy of allergic airway inflammation.
Key words
asthma /
vitamin E /
gamma-tocopherol /
eotaxin
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