Changes in expressions of vascular endothelial growth factor and inducible nitric oxide synthase protein in neonatal rats with white matter damage.

CHANG Xiao; TANG Cheng-he; CAO Yin-li; SHANG Yun; SHI Ji-peng; JIN Jian-yun.

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Chinese Journal of Child Health Care ›› 2013, Vol. 21 ›› Issue (3) : 276-279.

Changes in expressions of vascular endothelial growth factor and inducible nitric oxide synthase protein in neonatal rats with white matter damage.

CHANG Xiao¹,TANG Cheng-he¹,CAO Yin-li¹,SHANG Yun¹,SHI Ji-peng¹,JIN Jian-yun².

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Abstract

Objective To observe the expressions of vascular endothelial growth factor (VEGF) and inducible nitric oxide synthase(iNOS) protein in the brains of premature rats to moderate hypoxia-ischemia (HI),in order to explore possible relationships with white matter damage(WMD). Methods The 3 days old rats were randomly divided into experiment group and sham operation group.The pups were perfused at 12,24,48,72 h and 7 d of recovery from HI.Immunohistochemical technical was applied to investigate the changes of expressions of VEGF and iNOS in periventricular rats white matter tissues. Results In the experiment group,the expression of VEGF in periventricular rats white matter started to increase at 12 h and reached the peak at 24 h.The expression of iNOS was up-regulated at 12 h and peaked at 72 h after HI. Conclusion HI could induce the prominent increase in the expressions of VEGF and iNOS in white matter of the neonatal rats' brain,and may be involved in the pathophysiological process in the WMD.

Key words

white matter damage / hypoxia-ischemia / vascular endothelial growth factor / inducible nitric oxide synthase / neonatal rat

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CHANG Xiao,TANG Cheng-he,CAO Yin-li,SHANG Yun,SHI Ji-peng,JIN Jian-yun.. Changes in expressions of vascular endothelial growth factor and inducible nitric oxide synthase protein in neonatal rats with white matter damage.[J]. Chinese Journal of Child Health Care. 2013, 21(3): 276-279

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