Objective To investigate the expression of Omi/HtrA2 and cell apoptosis in brain of neonatal rats after asphyxia and to evaluate the effects of erythropoietin (EPO) on it. Methods These SD rats aged 7 days were randomly divided into the following groups:control group,asphyxia group and EPO treatment group.Animals of asphyxia group and EPO treatment group were reproduced by normobaric asphyxia,then EPO treatment group immediately injected the rhEPO (5 000 U/kg) in intraperitoneal immediately after asphyxia,the control group and asphyxia group were injected the same volume of 0.9% sodium chloride,then the brain tissues from the rats in the both groups were taken at 6,12,24,48 and 72 hours after the asphyxia.The pathological changes of brain tissue observed by HE staining ,the apoptotic cells were detected by the TUNEL staining method and the Omi/HtrA2 protein expression was detected with the immunhistochemistry method. Results In asphyxia groups,the number of apoptotic cells and the expression of Omi/HtrA2 were higher than those in control group at each time group (P<0.05).But compared with asphyxia group,the number of apoptotic cells were higher and the expressions of Omi/HtrA2 were significantly decreased in EPO treatment group,and the differences were significant(P＜0.05),but all parameters were not restored to the level of control group(P＜0.05). Conclusion EPO can reduce the number of apoptotic cells by inhibiting the expression of Omi/HtrA2 in brain,and thus can play a protective role in asphyxia brain damage of neonatal rats.
Key words
erythropoietin /
asphyxia /
neonatal rat /
apoptosis /
Omi/HtrA2
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