Objective To examine the relationships among p53 gene polymorphisms,environmental factors,and low birth weight infants. Methods A case-control study was conducted to collect the data of mothers and newborns,according to birth weight and gestational age,the infants were divided into healthy control group and low birth weight group (including preterm infants group and small for gestational age group).DNA from peripheral blood samples was examined by polymerase chain-restriction fragment length polymorphism (PCR-RFLP) method to detect the two p53 gene polymorphic situs p53codon72,p53PIN3 (rs1042522,rs17878362) and compared the genotype and allele frequency distribution in three groups. Results 1)Multivariate unconditional logistic regression analysis showed that the occurrence of low birth weight infants were associated with fetal gender,maternal education level,prenatal care and place of residence,and the regression coefficients were -0.710,1.055,0.825,-0.676,respectively.2)There was significant difference in the total distribution frequency of p53codon72 situs(χ2=19.182,P=0.001),but no association was detected between the low birth weight group and the healthy control group in p53PIN3 situs(χ2=0.566,P=0.754).The distribution frequencies of Pro/Pro genotype in p53codon72 situs in preterm infants group and small for gestational age group were significantly higher than those in normal birth weight infants group,individuals with the homozygous Pro/Pro genotype had increased risks of low birth weight[(OR=2.317,95%CI:1.290~4.162,P=0.002),(OR=2.805,95%CI:1.599~4.919,P=0.000)]. Conclusions Genetic and environmental factors and their interactions plays an important role in the incidence of low birth weight infants.p53codon72 situs polymorphism is related to low birth weight,and individuals allele of the p53 gene are at increased risk of low birth weight.
Key words
P53 gene polymorphism /
environment factors /
polymerase chainrestriction fragment length polymorphism /
low birth weight infants /
newborn
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