Objective Learning and memory abilities of rats were observed damaged in the Morris water maze,after expressions of Homer 1a in their brain interfered by RNA interference (RNA interference,RNAi).Expressions of mGluR 5 in their brain were detected to explore the molecular mechanism of learning and memory impairment after expressions of Homer 1a in their brain interfered by RNAi. Methods 24 Sprague Dawley (SD) rats were randomly divided into two groups:Homer 1a RNAi group (RNAi group,n=12) and control group (Nc group,n=12),and injected into the intracerebroventricular of the rats with Homer 1a RNAi lentivirus or nonsense lentivirus,respectively.Seven days later,completed the behavior tests (Morris Water Maze),mRNA and protein expressions of mGluR5 in some regions (prefrontal cortex,striatum,hippocampus) were analyzed using Quantitative PCR and Western blot. Result mGluR5 mRNA and protein expression in the striatum and hippocampus of RNAi group compared with the control group were significantly lower (striatum:mRNA t=6.67,protein t=3.10;hippocampus:mRNA t=3.08;protein t=2.89,all P<0.05 or <0.01),while there were no significant differences in the prefrontal(mRNA t=1.58,protein t=1.66,all P>0.05). Conclusion Homer 1a RNAi rats exhibit learning and memory impairment,and the expressions of Homer 1a and its related gene mGluR5 are changed,suggesting that mGluR5,as Homer 1a downstream gene,is involved in the mechanisms of learning and memory impairment after expressions of Homer 1a in their brain interfered by RNAi.
Key words
RNA interference /
ADHD /
Homer 1a /
metabotropic glutamate receptor 5 /
learning and memory
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