Changes in global DNA methylation and the effects of n-3 polyunsaturated fatty acids in diet-induced obese mice

SHEN Wen-wen; FAN Chao-nan; XIA Lu-lu; WANG Cui; DONG Hua; QI Ke-min

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Chinese Journal of Child Health Care ›› 2012, Vol. 20 ›› Issue (4) : 333-335.

Changes in global DNA methylation and the effects of n-3 polyunsaturated fatty acids in diet-induced obese mice

SHEN Wen-wen,FAN Chao-nan,XIA Lu-lu,WANG Cui,DONG Hua,QI Ke-min

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Abstract

【Objective】 The aim of this study is to investigate the changes in global DNA methylation and the effects of n-3 polyunsaturated fatty acids (n-3 PUFAs) in obesity. 【Methods】 Thirty 3~4 week-old C57BL/6J male mice were randomly divided into 3 groups.Mice were fed with two types of high-fat diet (containing 34.9% fat providing 60% of total energy) with n-6 PUFAs (fat from sunflower oil) and n-3 PUFAs (fat from fish oil) respectively to induce obesity for 14 weeks,with a normal-fat diet (4.3% fat providing 10% of total energy)(fat from sunflower oil) as lean control.After fasted for 12 hours,mice were sacrificed and the epididymal fat was collected.Global DNA methylation and the expression of DNA methyltransferases (DNMTs) from the adipose tissue were measured by enzyme-linked immunosorbent assay. 【Results】 As compared to the lean control mice,the rates of global DNA methylation in adipose tissue were significantly increased in the two groups of diet-induced obese (DIO) mice.If the two groups of DIO mice were compared,the n-3 PUFAs in diets had inhibitory effects on the global DNA methylation.The expressions of DNMT1,DNMT3a and DNMT3b in the adipose tissue were higher in DIO mice fed the n-6 PUFAs diet compared to the normal-fat diet,whereas no changes were shown in the expression of these DNMTs in DIO mice fed the n-3 PUFAs diet. 【Conclusion】 The global DNA methylation in the adipose tissue of obesity increases and dietary fish oil n-3 PUFAs have inhibitory effects on DNA methylation.

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DNA methylation / DNA methyltransferases / n-3 polyunsaturated fatty acids / obesity / mice

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SHEN Wen-wen,FAN Chao-nan,XIA Lu-lu,WANG Cui,DONG Hua,QI Ke-min. Changes in global DNA methylation and the effects of n-3 polyunsaturated fatty acids in diet-induced obese mice[J]. Chinese Journal of Child Health Care. 2012, 20(4): 333-335

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