Changes of superoxide dismutase,malonyldialdehyde,and pathology in the infant rats with hypoxic-ischemic brain damage and protective effect of hyperbaric oxygen

CHEN Jing; CHEN Yan-hui; LV Hong-yan

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Chinese Journal of Child Health Care ›› 2012, Vol. 20 ›› Issue (12) : 1094-1097.

Changes of superoxide dismutase,malonyldialdehyde,and pathology in the infant rats with hypoxic-ischemic brain damage and protective effect of hyperbaric oxygen

CHEN Jing¹,CHEN Yan-hui²,LV Hong-yan²

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Abstract

【Objective】 To explore the effects of hyperbaric oxygen (HBO) on the changes of superoxide dismutase(SOD),malonyldialdehyde (MDA) and pathology in brain tissue of infant rats with hypoxic-ischemic brain damage (HIBD). 【Methods】 The rat model of HIBD was made by the method of Bjelke and divided randomly into two groups (n=30)-HIBD group and HBO-treated HIBD group.Another 60 rats underwent sham-operation and were also divided randomly into HBO-treated the control group and the control group.After 24h of the operation,the rats of the HBO-treated groups received HBO (2ATA,1h/d) for 14 days.The rats were killed at postnatal 12h and 15d to detect the level of SOD and MDA in rat brain.HE staining and Nissl stain were employed to observe the pathological change and the number of neurons in hippocampus of rats when rats were 15 days old. 【Results】 In HIBD+HBO group,the level of SOD[ (90.43±10.83) U/mgprot] was higher than that in HIBD group [(74.18±8.52) U/mgprot]; but the level of MDA[ (16.76±3.80) nmol / mgprot ]was lower than that in HIBD group[(28.05±4.28)nmol/ mgprot].Surviving neurons of the HIBD+HBO rats[(122±10.52) cells /0.2 mm²] were more than the HIBD rats[(92± 6.51) cells/0.2 mm²] by Nissl's staining. 【Conclusion】 HBO might be useful to protect brain of neonatal rat against hypoxic-ischemic damage by resisting free redical,reducing brain edema and reducing neuron death.

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rats / hypoxic-ischemic brain damage / hyperbaric oxygen / oxygen free radicals / neuron death

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CHEN Jing,CHEN Yan-hui,LV Hong-yan. Changes of superoxide dismutase,malonyldialdehyde,and pathology in the infant rats with hypoxic-ischemic brain damage and protective effect of hyperbaric oxygen[J]. Chinese Journal of Child Health Care. 2012, 20(12): 1094-1097

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