【Objective】 To explore the effect of NYGGF4 (PID1) gene over-expression on mitochondrial metabolism in adipocytes. 【Methods】 3T3-L1 preadipocytes transfected with either a NYGGF4 expression vector or an empty expression vector were induced to differentiate into adipocytes.The expression levers of metabolic enzymes in mitochondria-Hexokinase (HKI),Acetyl-CoA (ACC),citrate synthase (CS),Carnitine palmitoyl-transferase 1 (CPT1),Cytochrome C (Cyc-C) were detected by Real-time PCR. 【Result】 NYGGF4 over-expression was significantly lower level of metabolic enzymes in mitochondria-HKI,ACC,CS,CPT1,Cyc-c in adipocytes than control (NC) cells. 【Conclusion】 In adipocytes with NYGGF4 gene over-expression,mitochondria metabolic enzymes mRNA is down- regulated.It could implicate that mitochondrial metablism is influenced in adipocytes with NYGGF4 gene over-expression.
Key words
NYGGF4 gene /
mitochondria /
mitochondrial metabolism /
3T3-L1 adipocytes
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