【Objective】 The bioinformatics analysis about hsa-miR-378 was performed,including transcription regulation prediction and function analysis of target genes,in order to lay foundation and provide the basis for the further studies in human preadipocytes differentiation. 【Methods】 Online tools,such as NCBI Mapviewer,UCSC Genome Browser and Promoter scan,were used for prediction of the CpG island,transcription start position (TSS),transcription factor binding sites (TFBS),etc.TargetScan,PicTar and miRanda were used to predict target genes of hsa-miR-378.The intersection of the three results and validated targets from DIANA LAB-TarBase 6.0 datebase were analyzed by gene ontology and pathway analysis. 【Results】 hsa-miR-378 might had an independent promoter with C/EBP β transcription factor binding sites.The gene ontology analysis showed the predicted target genes were enriched in transcriptional regulation,protein modification,cell differentiation and other biological processes(P<0.01).The pathway analysis showed the target genes mainly involved in 4 signaling pathways such as TGF-β signaling pathway,Wnt signaling pathway,and 8 disease pathways such as thyroid cancer,systemic lupus erythematosus(P<0.05). 【Conclusion】 The analysis about transcriptional regulatory elements and target genes of hsa-miR-378 lie the foundation for subsequent research about its function and regulatory mechanism in human preadipocytes.
Key words
hsa-miR-378 /
microRNA /
bioinformatics analysis /
adipocytes differentiation /
obesity
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