【Objective】 To study the reference value of serum alkaline phosphatase(ALP) on minor aged 0～16 years old in Shenyang area. 【Methods】 Applying SPSS 17.0, ALP of 1 447 minor under 16 years old were analyzed by grouping.The reference value of minor serum ALP was set up by statistic methods. 【Results】 The reference value of minor serum ALP under 16 years old was set up.ALP of minor were higher than those of adult. The difference of ALP between male and female above 6 years old were significant,but were not under 6 years old. The ALP between male and female were superposed twice. The collectivity tendency were those ALP were higher in newborn and above 3 years old,but were declined under 3 years old and adolescence. 【Conclusion】 It is essential to set up the reference value of minor serum ALP.