【Objective】 To investigate the effects of n-3 polyunsaturated fatty acids(n-3 PUFAs) on the mRNA expression of satiety regulators in diet-induced obese (DIO) mice. 【Methods】 Three to four-week-old male C57BL/6J mice were randomly divided into 3 groups with 15 cases in each group. There mice were fed with two types of high-fat diet(34.9% fat providing 60% of total energy) with n-6 PUFAs(originated from sunflower oil) and n-3 PUFAs(originated from fish oil) respectively to induce obesity, with a normal-fat diet(4.3% fat providing 10% of total energy) as lean control. After fasted for 12 hours, blood samples were drawn, and brains and epididymal fat were collected. Plasma leptin concentrations were measured by enzyme-linked immunosorbent assay. The mRNA expressions of leptin in fat and leptin receptor(leptin-R), neuropeptide Y(NPY) and proopiomelanocortin(POMC) in the hypothylamus were assayed by real-time RT-PCR. 【Results】 The body weight and plasma leptin concentration in both DIO mice were significantly increased compared to the lean control mice, however the changes in the DIO mice with the n-3 PUFA diet were much smaller than the DIO mice with the n-6 PUFA diet. As compared to the lean control mice, the mRNA expressions of leptin, leptin-R and POMC were higher and those of NPY were lower in DIO mice with the n-6 PUFA diet, whereas no changes were shown for the four genes' expression in the DIO mice with the n-3 PUFA diet. 【Conclusion】 Dietary fish oil n-3 PUFAs may play some positive roles by antagonizing the abnormal expressions of the satiety regulators in DIO mice.
Key words
n-3 polyunsaturated fatty acids /
obesity /
mice /
leptin /
neuropeptide Y /
proopiomelanocortin
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