【Objective】 To explore the frequency distribution of apolipoproteinE(ApoE) gene polymorphism of obesity children and its effection on lipid levels in plasma. 【Methods】 Using DNA sequencing method to detect the apolipoproteinE gene polymorphism of 100 obesity children and 100 normal children of control group. Moreover, total cholesterol(TC), triglyceride(TG), high-density lipoprotein cholesterol(HDL-C), low-density lipoprotein cholesterol(LDL-C), apolipoprotein A(apoA) and apolipoprotein B(apoB) were detected with the 12 h fasting blood. Observing the distribution of different genotypes and allele, and analysis of its correlation with lipid. 【Results】 E3/3 genotype frequence(＞70%) of ApoE was quite higher than E3 heterozygous (E2/3 and E3/4＞20%) and E2/2and E4/4; compared to the control group, E2 allele of the experimental group had higher level of TG(P＜0.05), E3 allele had higher level of TG, TC, LDL and lower level of HDL, ApoA(P＜0.01), while E4 allele had higher level of TG, AopB and lower level of HDL, ApoA(P＜0.05), the differences had statistical significance; in experimental group, compared to E3 allele, the child who carry E2 allele had lower level of TC, LDL and ApoB(P＞0.05), E4 allele has higher level of TG, ApoB and lower HDL(P＜0.05); the obesity children who carry different allele had different dyslipidemia rate, it was E4＞E3＞E2(P＜0.01). 【Conclusion】 The apoE3/3 is the most common genotype, and ApoE4 are correlated with dyslipidemia of obesity children and is one of the risk genes of dyslipidemia.
Key words
apolipoproteinE /
obesity children /
polymorphism /
lipid
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