【Objective】 To investigate the pathogens and drug tolerance of newborn dacryocystitis in order to provide evidence of clinical drug use. 【Method】 From August 2009 to October 2010, 220 cases of newborn dacryocystitis were analyzed for bacterial culture and drug sensitivity tests in children's hospital of Fudan university. 【Results】 Of the 220 samples with newborn dacryocystitis, pathogens detecting rate was 91.4％(201 cases).44.7%(93 cases) strains were gram-negative, the first three kinds of isolated bacteria were escherichia col, klebsiella pneumonia and haemophilus influenza. 52.9%(110 cases) strains were gram-positive, the first three kinds of isolated bacteria were staphylococcus epidermidis, streptococcus viridans and staphylococcus aureus. 2.4%(5 cases) were epiphyte. Gram-negative bacteria were more sensitive to levofloxacin and ciprofloxacin, the sensitivity rate was 93.5% and 90.3%. Gram-positive bacteria were more sensitive to levofloxacin and tobramycin, the sensitivity rate was 91.8% and 90.0%. Both gram-negative bacteria and gram-positive bacteria's sensitivity to erythromycin were lowest. 【Conclusion】 Gram-positive bacteria's detecting rate is higher than gram-negative bacteria's of newborn dacryocystitis, both are more sensitive to the types of antibiotics of quinolones and aminoglycosides.