【Objective】 To establish an animal model of rickets that sensitized for measuring their concentration include 25-(OH)D3、IgE、IFN-γ、IL-4 in venous blood after supply vitamin D, as a reference in prophylaxis and treatment of rickets and systemic anaphylaxis disease in pediatry. 【Methods】 The SD rats model of rickets that sensitized were divided into 3 groups in experiment include 3×105 U doses of vitamin D group (7 rats),6×105 U doses of vitamin D group (7 rats) 、control group(6 rats), all the way to a muscle column for experimental groups ,the method include of Elisa and flow cytometry used to test allergy and immunity target of rats after raising 20 days. 【Results】 The experimental groups compared with the control group, 3×105 U doses of vitamin D decreased the allergy target -IgE of the SD rats(P＜0.01), but 6×105 U doses of vitamin D made IgE increased(P＜0.01),the UR value of IFN-γ of small dose group increased(P＜0.05),but still under normal rats, the UR value of IL-4 decreased; the UR value of IFN-γ of 6×105 U doses group was not only ascend, but also descend evidently(P＜0.05),but the UR value of IL-4 increased(P＜0.05). 【Conclusion】 3×105 U doses of vitamin D could improve this unbalance , but 6×105 U doses can intensify Th1/Th2 unbalance of allergy body, further increase the physical allergy .
Key words
the rickets rat after sensitization /
vitamins D /
IFN-γ /
IL-4 /
Th1/Th2
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