【Objective】 To investigate the potential roles of budesonide in asthma treatment, the pathological changes of asthmatic lung tissue and expressions of tumor necrosis factor receptor associated factor 2(TRAF2) were performed in rat asthma model. 【Methods】 The rats of asthma model were randomly divided into three groups on average, including asthma group, control group and budesonide treated group. The changes of lung inflammation were observed by hematoxylin eosin staining.The expressions of TRAF2 proteins were detected by immunohistochemical method either. 【Results】 There were significant differences between in asthma group and in control group about changes of lung inflammation and generral conditions of hairs, weight, activity, and so on . while they were significantly decreased by budesonide.Moreover, the expressions of TRAF2 proteins in asthma group (0.317±0.041 optical density)at bronchial wall were significantly higher than those in control group(0.220±0.057 optical density)(P＜0.01), and the expressions were dramaticly decreased in budesonide treated group(0.236±0.033 optical density) when compared to asthma group(P＜0.01). But there was no significant difference of TRAF2 protein expressions between in budesonide treated group and control group( P>0.05). 【Conclusions】 Levels of TRAF2 are increased in asthmatic rat, it may be as a potential role in pathogenesis of asthma inflammation. The function of budesonide on regulating TRAF2 levels may be partly through TRAF2 signal transduction pathway.
Key words
asthma /
tumor necrosis factor receptor associated factor /
immunohistochemisty /
rat
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