【Objective】 To evaluate the iodine nutritional status and its influencing factors among infants in some regions of Jiangsu province, aims to make recommendation for suitable iodine intake during this locality. 【Methods】 Utilizing a stratified random sampling design, a total of 2 329 healthy infants were recruited from five surveillance areas(Nanjing、Nantong,Changzhou and two major outskirt rural regions). For every participant 5 mL spot urine sample was collected to assay iodine concentration. A dietary questionnaire was completed and anthropometric parameters(height, weight) were measured ,then the correlations between urinary iodine levels and its influencing factors were analyzed. 【Results】 1)The median urine iodine concentration of the infants was 200 μg/L, and the distribution of urinary iodine concentration (UIC) range from 25% to 75% was 100~250 μg/L.1.3% of which was lower than 50 μg/L and 5.2% was higher than 300 μg/L.The rate of UIC of 50~99 μg/L,100~300 μg/L was 10.9%,82.7%,respectively.2)Urinary iodine concentration's constituent ratio were differ significantly both in different months(χ²=19.870,P<0.05) and different regions(χ²=60.355,P<0.001).The rate of UIC more than 300 μg/L and less than 100 μg/L in urban were higher than rural(χ²=33.208,P<0.001).3)The multiple conditional logistic regression analysis was used to explore the relationship between urinary iodine and general conditions' of infant (age,regions and intake of salt sodium,et al),and it was found that urinary iodine was related to age、residential area and intake of salt sodium. 【Conclusions】 The average iodine nutritional status of infants are at the appropriate level in Jiangsu province,however,there are also iodine deficiency and excess in some places of Jiangsu province; The distribution of urinary iodine is differ in different regions, and a higher proportion of iodine deficiency and excess in urban especially in Nanjing city.