【Objective】 To understand the quality of sterilizing and hygiene among pre-schools of migrant workers' children in Minhang district in order to provide scientific evidences for preventing infectious diseases among those children. 【Methods】 Using field investigation method quality of hygiene investigated among pre-schools of migrant workers' children in Minhang District. Using sampling method the atmosphere, hands of staff, surface of facilities and dishware related to those pre-schools of migrant workers' children monitored . 【Results】 19 pre-schools of migrant workers' children were not legally approved by government. The total number of children exceeded 150 within those 10 pre-schools of migrant workers' children. The average numbers of children were over 40 of each class among 16 pre-schools of migrant workers' children. The ratio between staff and children was 35 to 1. There were no records of sterilizing about relevant staff among half schools sampled. The towels and dishware were misused in some schools. The qualified rate of sterilizing was 75.22%, the atmosphere was 52.63%, the hands of staff was 59.26%, the dishware was 80.00%, the surface of facilities was 91.49%. 【Conclusions】 There are weaknesses with the layout facilities, staff resources, managing systems of health. The quality of sterilizing is not good enough. It is necessary to implement prevention strategy in order to thoroughly solve those problems existed in pre-schools of migrant workers' children in Minhang District.