Effects of exercise on liver metabolism in obese mice through integrated proteomics and metabolomics analysis

WANG Yuhan; WU Haojie; SHI Lin; ZHANG Ting

doi:10.11852/zgetbjzz2025-0588

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Chinese Journal of Child Health Care ›› 2026, Vol. 34 ›› Issue (1) : 17-22. DOI: 10.11852/zgetbjzz2025-0588

Column on physical activity and childhood weight management

Effects of exercise on liver metabolism in obese mice through integrated proteomics and metabolomics analysis

WANG Yuhan, WU Haojie, SHI Lin, ZHANG Ting

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Abstract

Objective To investigate the effects of combined exercise and dietary intervention on liver proteins and metabolites in obese mice, so as to provide a theoretical basis for understanding the molecular mechanisms by which this intervention ameliorates obesity. Methods Thirty male C57BL/6 mice were randomly assigned to three groups: control group, obesity group, and combined invention group, with 10 mice in each group.The control group received a standard diet for 12 weeks, whereas the obesity group received a high-fat diet for the same duration.Following 8 weeks of high-fat diet feeding, the combined invention group received 4 weeks of regular feed and moderate intensity exercise intervention.Proteomics and metabolomics analyses were performed to evaluate protein expression levels and metabolite content in liver tissues across the groups at the end of 12 weeks.Bioinformatics analyses were conducted to identify differences among the groups. Results In comparison to the obesity group, the combined intervention group demonstrated significant upregulation of 157 proteins and 79 metabolites, as well as significant downregulation of 104 proteins and 48 metabolites.Multi-omics analysis revealed that combined exercise and diet intervention primarily influenced liver metabolism by modulating retinol, fatty acid, and linoleic acid metabolism, and bile secretion. Conclusions Combined exercise and dietary intervention significantly reduce body weight in obese mice, accompanied by alterations in hepatic retinol metabolism, bile acid secretion, and lipid-related metabolic pathways.These findings suggest that these metabolic pathways may be involved in the metabolic remodeling induced by the intervention.

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combined exercise and diet intervention / obesity / bile acids / retinol metabolism

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WANG Yuhan, WU Haojie, SHI Lin, ZHANG Ting. Effects of exercise on liver metabolism in obese mice through integrated proteomics and metabolomics analysis[J]. Chinese Journal of Child Health Care. 2026, 34(1): 17-22 https://doi.org/10.11852/zgetbjzz2025-0588

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