Objective To investigate the effects of Shenshao Capsule on serum inflammatory factors and learning-memory abilities in preterm offspring rats induced by intrauterine infection, in order to provide new insights for mitigating learning and memory impairments in preterm infants. Methods Sixty pregnant rats were randomly divided into two groups (experimental group and control group).From gestational day 1, the experimental group received a daily gavage of Shenshao Capsule solution (25mg/mL), while the control group received an equivalent volume of normal saline.Dosages were adjusted based on human-to-rat body surface area conversion.On gestational day 17, both groups were intrauterinely injected with 100μL of lipopolysaccharide (LPS, 25μg/μL) solution.On gestational day 18, 15 pregnant rats from each group were randomly selected and euthanized by cervical dislocation.ELISA was used to measure changes in serum IL-4 and IL-10 levels in the offspring.The remaining 15 pregnant rats in each group were allowed to deliver naturally.After observing pregnancy outcomes, 10 offspring from each group were randomly selected for Morris water maze testing at one month of age to assess learning and memory abilities. Results Compared with the control group, the experimental group exhibited significantly lower preterm birth rate (χ2=14.122),and decreased stillbirth rate (χ2=7.266),higher live birth count (t=5.386) (P<0.05).Serum IL-4 and IL-10 levels in the experimental offspring were significantly different from those in the control group (t=5.652, 6.273, P<0.05).In the navigation test, the experimental offspring adopted more effective strategies (χ2=2.568, P<0.05) and demonstrated significantly shorter mean escape latencies (F=64.32, P<0.05).In the spatial probe test, the experimental group spent significantly less time in the target quadrant (t=3.038, P<0.05). Conclusions Shenshao Capsule alleviates inflammatory responses in preterm offspring rats and improves their learning and memory capacities.
Key words
premature birth /
offspring /
learning-memory /
Shenshao Capsule
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