Correlation between gutmicrobiota and symptom severity in children with autism spectrum disorder

LI Dexin&gt;; CHEN Qiang&gt;; ZHUANG Zhicheng&gt;; CHEN Hong&gt;; YANG Bingjiao&gt;; JIANG Shunxiang&gt;; ZHOU Xiang&gt;

doi:10.11852/zgetbjzz2022-1252

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Chinese Journal of Child Health Care ›› 2023, Vol. 31 ›› Issue (5) : 497-501. DOI: 10.11852/zgetbjzz2022-1252

Original Articles

Correlation between gutmicrobiota and symptom severity in children with autism spectrum disorder

LI Dexin¹, CHEN Qiang¹, ZHUANG Zhicheng¹, CHEN Hong¹, YANG Bingjiao¹, JIANG Shunxiang², ZHOU Xiang¹

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Abstract

Objective To analyze structural characteristics of gut microbiota and its correlation with symptom severity in children with autism spectrum disorder (ASD), in order to provide new ideas for exploring pathogeny and targeted therapy. Methods A total of 107 ASD children in the Child Mental Health Department of Zhuhai Maternal and Child Health Hospital from March 2021 to September 2021 were selected as the ASD group, and 30 normal preschool children in a kindergarten were included as the control group.The severity of ASD symptoms was assessed using the Childhood Autism Rating Scale (CARS).Sample DNA was extracted after collecting fresh stools from both groups of children, and then the 16S rDNA V4 region of the bacteria was amplified and sequenced in high throughput. Results There was no significant difference between the alpha diversity index and beta diversity of the gut microbiota between children in ASD group and control group (P>0.05).At the phylum level, the relative abundance of the Proteobacteria in the ASD group was significantly lower than that in the control group (t=-3.103, P<0.05).At the family level, the dominant gut microbiota of children in ASD group included Tannerellaceae, Eggerthellaceae, Desulfovibrionales, Selenomonadaceae, Moraxellaceae, Bifidobacterium, while the dominant gut microbiota in normal group included Barnesiellaceae, Streptococcus, Barnesiella.At the genus level, the differences in the relative abundance of Anaerostipes, Collinsella, Blautia, and Megamonas in children with different severity of symptoms were statistically significant (P<0.05).Spearman correlation analysis showed a positive correlation of Blautia and Megamonas with CARS score in ASD children (r=0.213, 0.288, P<0.05). Conclusion Gut microbiota of ASD children is disturbed and its structure is strongly related to the severity of behavioral symptoms.

Key words

autism spectrum disorder / gut microbiota / high-throughput sequencing

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LI Dexin>, CHEN Qiang>, ZHUANG Zhicheng>, CHEN Hong>, YANG Bingjiao>, JIANG Shunxiang>, ZHOU Xiang>. Correlation between gutmicrobiota and symptom severity in children with autism spectrum disorder[J]. Chinese Journal of Child Health Care. 2023, 31(5): 497-501 https://doi.org/10.11852/zgetbjzz2022-1252

References

[1] Maenner MJ, Shaw KA, Baio J,et al.Prevalence of autism spectrum disorder among children aged 8 years - autism and developmental disabilities monitoring network, 11 sites, united states, 2016[J].MMWR Surveill Summ, 2020,69(4):1-12.
[2] Vuong HE, Hsiao EY.Emerging roles for the gut microbiome in autism spectrum disorder[J].Biol Psychiatry, 2017,81(5):411-423.
[3] Hsiao EY, McBride SW, Hsien S,et al.Microbiota modulate behavioral and physiological abnormalities associated with neurodevelopmental disorders[J].Cell, 2013,155(7):1451-1463.
[4] 赵英欣, 张旭静, 郑毅.肠道微生物-大脑轴与孤独症谱系障碍[J].中华精神科杂志, 2019,52(3):224-227.
Zhao YX,Zhang XJ,Zheng Y.The gut microbe-brain axis and autism spectrum disorders[J].Chin J Psychiatry,2019,52(3):224-227.(in Chinese)
[5] 陈真竹, 任燕, 杨红.双相情感障碍与肠道菌群[J].中华行为医学与脑科学杂志, 2020,29(5):475-480.
Chen ZZ,Ren Y,Yang H.Bipolar disorder and intestinal flora[J].Chi J Behav Med Bra Sci, 2020,29(05):475-480.(in Chinese)
[6] Svoboda E.Could the gut microbiome be linked to autism?[J].Nature, 2020,577(7792 suppl):14-15.
[7] Yap CX, Henders AK, Alvares GA,et al.Autism-related dietary preferences mediate autism-gut microbiome associations[J].Cell, 2021,184(24):5916-5931,e5917.
[8] 黎萍, 王叶, 邢杰.肠道微生物与孤独症谱系障碍的研究进展[J].中国儿童保健杂志, 2016,24(12):1284-1286.
Li P,Wang Y,Xing J.Advances in the study of gut microbes and autism spectrum disorders[J].Chin J Child Health Care, 2016,24(12):1284-1286.(in Chinese)
[9] Kang DW, Park JG, Ilhan ZE,et al.Reduced incidence of prevotella and other fermenters in intestinal microflora of autistic children[J].PLoS One, 2013,8(7):e68322.
[10] Madra M, Ringel R, Margolis KG.Gastrointestinal issues and autism spectrum disorder[J].Child Adolesc Psychiatr Clin N Am, 2020,29(3):501-513.
[11] Kostiukow A, Poniewierski P, Daroszewski P,et al.Gastrointestinal disorders in children with autism spectrum disorder[J].Pol Merkur Lekarski, 2020,48(283):69-72.
[12] Yang J, Fu X, Liao X,et al.Effects of gut microbial-based treatments on gut microbiota, behavioral symptoms, and gastrointestinal symptoms in children with autism spectrum disorder: A systematic review[J].Psychiatry Res, 2020,293(11):113471.
[13] Wang L, Christophersen CT, Sorich MJ,et al.Low relative abundances of the mucolytic bacterium akkermansia muciniphila and bifidobacterium spp.in feces of children with autism[J].Appl Environ Microbiol, 2011,77(18):6718-6721.
[14] Buie T, Campbell DB, Fuchs GJ,et al.Evaluation, diagnosis, and treatment of gastrointestinal disorders in individuals with ASDs: A consensus report[J].Pediatrics, 2010, 125(Suppl 1):1-18.
[15] Chaidez V, Hansen RL, Hertz-Picciotto I.Gastrointestinal problems in children with autism, developmental delays or typical development[J].J Autism Dev Disord, 2014,44(5):1117-1127.
[16] 曹歆轶,林萍,蒋平,等.儿童孤独症谱系障碍胃肠道细菌学研究的系统综述[J].上海精神医学,2013,25(6):342-353.
Cao XY, Lin P, Jiang P,et al.Characteristics of the gastrointestinal microbiome in children with autism spectrum disorder:A systematic review[J].Shanghai Arch Psychiatry, 2013,25(6):342-353.(in Chinese)
[17] de Theije CG, Wopereis H, Ramadan M,et al.Altered gut microbiota and activity in a murine model of autism spectrum disorders[J].Brain Behav Immun, 2014,3(37):197-206.
[18] Vargas-Caraveo A, Sayd A, Maus SR,et al.Lipopolysaccharide enters the rat brain by a lipoprotein-mediated transport mechanism in physiological conditions[J].Sci Rep, 2017,7(1):13113.
[19] Li N, Yang J, Zhang J,et al.Correlation of gut microbiome between ASD children and mothers and potential biomarkers for risk assessment[J].Genomics Proteomics Bioinformatics, 2019,17(1):26-38.
[20] 程中乐, 魏薇, 王旭,等.孤独症模型大鼠及患儿肠道菌群、内毒素和肝功能关系的研究[J].中华神经医学杂志, 2016,15(7):705-709.
Chen ZL,Wei W,Wang X,et al.Study on the relationship between intestinal flora, endotoxin and liver function in rats and children with autism model[J].Chin J Neuro, 2016,15(7):705-709.(in Chinese)
[21] Tomova A, Husarova V, Lakatosova S,et al.Gastrointestinal microbiota in children with autism in Slovakia[J].Physiol Behav, 2015,138:179-187.
[22] 马冰洁, 梁晶晶, 静进.孤独症谱系障碍儿童肠道菌群与症状严重程度的关系研究[J].教育生物学杂志, 2020,8(1):30-35.
Ma BJ,Liang JJ,Jing J.A study of the relationship between intestinal flora and symptom severity in children with autism spectrum disorders[J].J Bio-edu, 2020,8(1):30-35.(in Chinese)
[23] 曾津, 危松青, 王曼知.孤独症谱系障碍患儿肠道菌群与症状严重程度相关性分析[J].精神医学杂志, 2020,33(1):62-65.
Zeng J,Wei SQ,Wang MZ.Correlation between intestinal flora and symptom severity in children with autism spectrum disorders[J].J Psychiatry.2020,33(1):62-65.(in Chinese)
[24] Settanni CR, Bibbò S, Ianiro G,et al.Gastrointestinal involvement of autism spectrum disorder: focus on gut microbiota[J].Expert Rev Gastroenterol Hepatol, 2021,15(6):599-622.
[25] Liu F, Li J, Wu F,et al.Altered composition and function of intestinal microbiota in autism spectrum disorders: A systematic review[J].Transl Psychiatry, 2019,9(1):43.
[26] Silva YP, Bernardi A, Frozza RL.The role of short-chain fatty acids from gut microbiota in gut-brain communication[J].Front Endocrinol (Lausanne), 2020,11(1):25.