Bioinformatics analysis of the regulation of miR-429 towards target genes in tic disorders

JIANG Jilong; CHEN Yanhui; HUANG Huifang; HUANG Yuxian

doi:10.11852/zgetbjzz2022-0746

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Chinese Journal of Child Health Care ›› 2023, Vol. 31 ›› Issue (3) : 268-273. DOI: 10.11852/zgetbjzz2022-0746

Original Articles

Bioinformatics analysis of the regulation of miR-429 towards target genes in tic disorders

JIANG Jilong, CHEN Yanhui, HUANG Huifang, HUANG Yuxian

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Abstract

Objective To predict tic disorders (TD) related target genes of microRNA (miRNA, miR)-429 by using bioinformatics analysis, and to take intersection between the target genes of miR-429 and TD related genes (TRGs), so as to further understand the impaction of miR-429 in TD. Method MiRbase was used to obtain the sequences of mature miR-429 in multiple species for conserved analysis.GeneCards database and DisGeNET database were used to acquire TRGs.MiRDB database and DIANA-microT were used to gain the target genes of miR-429.TD related target genes were obtained by intersection of the target genes gained by MiRDB database and DIANA-microT with TRGs.RNAhybird database was used to predict the sequences and minimum free energy (MFE) of hybridization.Functional enrichment analysis of TD related target genes was performed by DAVID database. Results Only 1 nucleotides among the 22 nucleotides of miR-429 was different among specie.A total of 9 genes were identified as TD related target genes.The sequences of hybridization of ASH1L and SLITRK1 with miR-429 did not include the nucleotide that differed among species, indicating that the two hybird pairs were more conserved.The first gene with the smallest MFE of hybridization were CHD2 (-25.0 kcal/mol).The first terms in each category of functional enrichment analysis were adult behavior of biological process, glutamatergic synapse of cell components and chromatin binding of molecular functions.No statistically significant pathway enrichment was obtained. Conclusion MiR-429 might impact behaviors in TD by regulating glutamatergic synapses via related genes, especially SLITRK1.

Key words

miR-429 / glutamatergic / SLITRK1 gene / bioinformatics analysis / tic disorders

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JIANG Jilong, CHEN Yanhui, HUANG Huifang, HUANG Yuxian. Bioinformatics analysis of the regulation of miR-429 towards target genes in tic disorders[J]. Chinese Journal of Child Health Care. 2023, 31(3): 268-273 https://doi.org/10.11852/zgetbjzz2022-0746

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