Objective To investigate the effects of different concentrations of mirtazapine on anxiety and depression like behavior induced by chronic restraint stress in adolescent mice, so as to provide data for the treatment of adolescent emotional disorders. Methods A total of 60 male C57 mice aged 3 - 4 weeks were randomly selected. The model group was under chronic restraint stress (CRS) 4 hours per day for 10 days. The treatment group was injected with low concentration (15 mg/kg) or high concentration (30 mg/kg) mirtazapine for 10 days. Anxiety like behavior was detected by open-field test (OFT), depression like behavior was detected by tail-suspension test (TST) and force swim test (FST), and serum mirtazapine level was detected by enzyme-linked immunosorbent assay. Results In the OFT experiment, the residence time of CRS mice in the central area was lower than that in the control group[(106.1±36.64)s vs. (182.5±38.69)s, P<0.01). After the application of high concentration mirtazapine (30 mg/kg), the time was extended to (186.1±23.43)s, which was close to the normal level. In the TST experiment, the resting time of CRS group was higher than that of control group[(176.2±104.5)s vs. (71.05±36.4)s, P<0.01]. After the application of high concentration mirtazapine (30 mg/kg), the time was shortened to (69.13±31.72)s (P > 0.05). In FST experiment, the floating time of CRS group was higher than that of control group[(304.6±87.96)s vs. (196.2±72.6)s, P<0.01]. After high concentration mirtazapine (30 mg/kg), the floating time was shortened to (188.8±49.05)s (P> 0.05). CRS resulted in that serum NE [(34.52±9.03)pg/ml] and 5-HT [(44.42±16.55)ng/ml] levels of mice in CRS group were lower than those of normal mice (P<0.01). After application of high concentration mirtazapine, it returned to the normal level (NE:(105.93±21.42)pg/mL,5-HT:(133.56±32.31)ng/mL. Conclusions CRS may lead to depression and anxiety like mood disorder in adolescent mice. Systemic chronic application of high concentration mirtazapine can improve the symptoms of anxiety and depression.
Key words
chronic restraint stress /
anxiety /
depression /
mirtazapine
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