Association between maternal intestinal microbiota in the third trimester of pregnancy and infant food allergy

WANG Shuo; LI Xin-yue; WEI Yuan; LIU Lu-yan; LI Zai-ling; ZHANG Hua

doi:10.11852/zgetbjzz2021-1749

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Chinese Journal of Child Health Care ›› 2022, Vol. 30 ›› Issue (9) : 941-946. DOI: 10.11852/zgetbjzz2021-1749

Original Articles

Association between maternal intestinal microbiota in the third trimester of pregnancy and infant food allergy

WANG Shuo^*, LI Xin-yue, WEI Yuan, LIU Lu-yan, LI Zai-ling, ZHANG Hua

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Abstract

Objective To investigate the relationship between maternal intestinal microbiota in the third trimester of pregnancy and infant food allergy, so as to provide new ideas for the association between microbial and food allergy. Methods A nested case-control study was adopted. Healthy pregnant women who had regular prenatal check-ups at Peking University Third Hospital from February 2018 to May 2020 and their newborns were selected to establish a maternal and infant cohort, of whom 135 mother-infant pairs met the criteria of this study and were followed up regularly. According to whether the infants had food allergy within 1 year old, 24 pairs were divided in the food allergy (FA) group and 44 pairs in the healthy control (HC) group. Fecal samples were collected from the mothers within 2 weeks before delivery and from the infants at 5 different time points after birth. These samples were analyzed by 16S rDNA sequencing. Results There was no significant difference in α diversity and β diversity of intestinal microbiota in the third trimester of pregnancy between the FA group and the HC group (P>0.05). Compared with the FA group, the relative abundance of Holdemania in maternal intestinal flora in the HC group was significantly increased(Z＝-2.103, P=0.035), while the relative abundance of Clostridium ⅪⅤa (Z＝-2.483, P=0.013), Clostridium Ⅳ (Z＝-2.154, P=0.031), Lactococcus (Z＝-2.147, P=0.032) and Parvimonas (Z＝-2.377, P=0.017) was significantly decreased. Among the intestinal microbiota of infants, there was no statistical significance in the difference between the main microbiota and the differential microbiota of maternal intestinal microbiota in late pregnancy at the same time point (P>0.05). Conclusions The relative abundance level of Holdemania in maternal intestinal microbiota in the third trimester of pregnancy is a potential predictor of food allergy in offspring, but is not associated with the relative abundance of Holdemania in offspring intestinal microbiota. Further studies on the mechanism of maternal intestinal microbiota in late pregnancy affecting offspring food allergy are warranted.

Key words

intestinal microbiota / the third trimester of pregnancy / infant / food allergy / 16S rDNA sequencing

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WANG Shuo, LI Xin-yue, WEI Yuan, LIU Lu-yan, LI Zai-ling, ZHANG Hua. Association between maternal intestinal microbiota in the third trimester of pregnancy and infant food allergy[J]. Chinese Journal of Child Health Care. 2022, 30(9): 941-946 https://doi.org/10.11852/zgetbjzz2021-1749

References

[1] Sicherer SH, Sampson HA. Food allergy[J]. J Allergy Clin Immunol, 2010,125(Suppl 2):116-125.
[2] Meyer R, Fox AT, Chebar Lozinsky A, et al. Non-IgE-mediated gastrointestinal allergies-Do they have a place in a new model of the Allergic March[J]. Pediatr Allergy Immunol,2019,30(2):149-158.
[3] Meyer R, Godwin H, Dziubak R, et al. The impact on quality of life on families of children on an elimination diet for non-immunoglobulin E mediated gastrointestinal food allergies[J]. World Allergy Organ J,2017,10(1):8.
[4] Sicherer SH, Sampson HA. Food allergy:A review and update on epidemiology, pathogenesis, diagnosis, prevention, and management[J]. J Allergy Clin Immunol,2018,141(1):41-58.
[5] Shu SA, Yuen AWT, Woo E, et al. Microbiota and food allergy[J]. Clin Rev Allergy Immunol,2019,57(1):83-97.
[6] Iweala OI, Nagler CR. The microbiome and food allergy[J]. Annu Rev Immunol,2019,37:377-403.
[7] 屠文骆,陈先俊,徐建萍,等. 孕晚期孕妇肠道菌群对子代婴儿免疫系统发育的影响[J]. 广东医学, 2021,42(10):1207-1210.
[8] Abbring S, Engen PA, Naqib A, et al. Raw milk-induced protection against food allergic symptoms in mice is accompanied by shifts in microbial community structure[J]. Int J Mol Sci,2021,22(7):3417.
[9] Wang Y, Qian PY. Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies[J]. PLoS One,2009,4(10):e7401.
[10] Edgar RC. UPARSE:highly accurate OTU sequences from microbial amplicon reads[J]. Nat Methods,2013,10(10):996-998.
[11] Cole JR, Wang Q, Fish JA, et al. Ribosomal database project: data and tools for high throughput rRNA analysis[J]. Nucleic Acids Res,2014,42:633-642.
[12] Segata N, Izard J, Waldron L, et al. Metagenomic biomarker discovery and explanation[J]. Genome Biol,2011,12(6):R60.
[13] Gomez-Arango LF, Barrett HL, Wilkinson SA, et al. Low dietary fiber intake increases Collinsella abundance in the gut microbiota of overweight and obese pregnant women[J]. Gut Microbes,2018,9(3):189-201.
[14] Barrett HL, Gomez-Arango LF, Wilkinson SA, et al. A vegetarian diet is a major determinant of gut microbiota composition in early pregnancy[J]. Nutrients,2018,10(7):890.
[15] Best KP, Gold M, Kennedy D, et al. Omega-3 long-chain PUFA intake during pregnancy and allergic disease outcomes in the offspring:A systematic review and meta-analysis of observational studies and randomized controlled trials[J]. Am J Clin Nutr,2016,103(1):128-143.
[16] Chen CC, Chen KJ, Kong MS, et al. Alterations in the gut microbiotas of children with food sensitization in early life[J]. Pediatr Allergy Immunol,2016,27(3):254-262.
[17] Nylund L, Satokari R, Nikkilá J, et al. Microarray analysis reveals marked intestinal microbiota aberrancy in infants having eczema compared to healthy children in at-risk for atopic disease[J]. BMC Microbiol,2013,13:12.
[18] Vael C, Vanheirstraeten L, Desager KN,et al. Denaturing gradient gel electrophoresis of neonatal intestinal microbiota in relation to the development of asthma[J]. BMC Microbiol,2011,11:68.
[19] Frossard CP, Steidler L, Eigenmann PA. Oral administration of an IL-10-secreting Lactococcus lactis strain prevents food-induced IgE sensitization[J]. J Allergy Clin Immunol,2007,119(4):952-959.
[20] Shin HS, Eom JE, Shin DU, et al. Preventive effects of a probiotic mixture in an ovalbumin-induced food allergy model[J]. Microbiol Biotechnol,2018,28(1):65-76.
[21] Walker RW, Clemente JC, Peter I, et al. The prenatal gut microbiome:Are we colonized with bacteria in utero?[J]. Pediatr Obes,2017,12(Suppl 1):3-17.
[22] Thum C, Cookson AL, Otter DE, et al. Can nutritional modulation of maternal intestinal microbiota influence the development of the infant gastrointestinal tract?[J]. Nutr,2012,142:1921-1928.
[23] 刘明颖,李辉,施展,等. 晚孕期孕妇口服益生菌制剂对新生儿肠道菌群及脐血IL-4、IFN-γ水平影响的临床研究[J]. 中国妇幼保健,2012,27(6):923-925.
[24] Vuillermin PJ, Macia L, Nanan R, et al. The maternal microbiome during pregnancy and allergic disease in the offspring[J]. Semin Immunopathol,2017,39(6):669-675.
[25] Tapiainen T, Paalanne N, Tejesvi MV, et al. Maternal influence on the fetal microbiome in a population-based study of the first-pass meconium[J]. Pediatr Res,2018,84(3):371-379.
[26] Ferretti P, Pasolli E, Tett A, et al. Mother-to-infant microbial transmission from different body sites shapes the developing infant gut microbiome[J]. Cell Host Microbe,2018,24(1):133-145.
[27] Carr LE, Virmani MD, Rosa F, et al. Role of human milk bioactives on infants' gut and immune health[J]. Front Immunol,2021,12:604080.
[28] Arpaia N, Campbell C, Fan X, et al. Metabolites produced by commensal bacteria promote peripheral regulatory T-cell generation[J]. Nature,2013,504(7480):451-455.
[29] Holder B, Jones T, Sancho Shimizu V, et al. Macrophage exosomes induce placental inflammatory cytokines: A novel mode of maternal-placental messaging[J]. Traffic,2016,17(2):168-178.