Characteristics of intestinal flora in infants aged 0 to 36 months in Beijing

YU Xiao-ran*; YAN Yin-kun*; HUANG Yi-wen; CHENG Hong; YAN Qi; WAN Nai-jun; LI Li-hua; REN Xia; XIE Xiang-hui; MI Jie

doi:10.11852/zgetbjzz2021-1686

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Chinese Journal of Child Health Care ›› 2022, Vol. 30 ›› Issue (2) : 124-129. DOI: 10.11852/zgetbjzz2021-1686

Original Articles

Characteristics of intestinal flora in infants aged 0 to 36 months in Beijing

YU Xiao-ran^*, YAN Yin-kun^*, HUANG Yi-wen, CHENG Hong, YAN Qi, WAN Nai-jun, LI Li-hua, REN Xia, XIE Xiang-hui, MI Jie

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Abstract

Objective To understand the changing characteristics of the intestinal flora of infants aged 0 to 36 months, and to explore the effect of exclusive breastfeeding on the colonization of intestinal flora from 0 to 36 months of age. Methods From January to April 2021, 291 healthy singleton infants and toddlers aged 0 to 36 months were enrolled in this study, and their fresh stools were collected in Haidian, Changping and Tongzhou districts of Beijing.The 77 infants aged 6 to 36 months were divided into the continuous breastfeeding group and the weaned group according to whether they were exclusive breastfeeding or not, then the effect of exclusive breastfeeding on the subsequent intestinal flora was analyzed.The 16S rRNA V4 region was sequenced using Illumina Novaseq 6000 sequencing platform. Results 1) The diversity and species composition of intestinal flora in infants changed with age, and Shannon index showed an increasing trend with age.At the phylum level, the intestinal flora was mainly composed of Proteobacteria, Firmicutes, Actinobacteria and Bacteroidetes.The abundance of Proteobacteria was the highest in newborns, but Firmicutes gradually replaced Proteobacteria as the dominant bacteria with the increase of age.2) If infants were exclusively breastfed before 6 months old, then the Shannon index and Observed-OTUs index after weaning of infants aged 6 to 36 months were higher than those continuously breastfed (Z=-2.400, P=0.015).Among weaned infants aged 6 to 36 months, the Shannon index of intestinal flora was higher in infants exclusively breastfed before 6 months old than that in infants mixed-fed before 6 months old (Z=-2.500, P=0.011). Conclusion The diversity and composition of the intestinal flora of infants vary significantly with age, and whether exclusive breastfeeding at 0 to 6 months old affects the diversity of intestinal flora in infants.

Key words

intestinal flora / high-throughput sequencing / exclusive breastfeeding / infants

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YU Xiao-ran^*, YAN Yin-kun^*, HUANG Yi-wen, CHENG Hong, YAN Qi, WAN Nai-jun, LI Li-hua, REN Xia, XIE Xiang-hui, MI Jie. Characteristics of intestinal flora in infants aged 0 to 36 months in Beijing[J]. Chinese Journal of Child Health Care. 2022, 30(2): 124-129 https://doi.org/10.11852/zgetbjzz2021-1686

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