Genome-wide changes of H2BK120ub1 inmouse neural tube defect embryos induced by retinoic acid

WANG Shan; HE Xue-jia; CHENG Xi-yue; LIN Ye; PEI Pei; ZHAN Xiao-jun

doi:10.11852/zgetbjzz2021-1609

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Chinese Journal of Child Health Care ›› 2022, Vol. 30 ›› Issue (7) : 731-736. DOI: 10.11852/zgetbjzz2021-1609

Basic Experimental Articles

Genome-wide changes of H2BK120ub1 inmouse neural tube defect embryos induced by retinoic acid

WANG Shan^*, HE Xue-jia, CHENG Xi-yue, LIN Ye, PEI Pei, ZHAN Xiao-jun

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Abstract

Objective To analyze the difference in genome-wide profile of H2BK120 monoubiquitination (H2BK120ub1) modification in embryonic brain tissue between E10.5 day normal mice and mice with retinoic acid (RA)-induced neural tube defects (NTDs) by chromatin immunoprecipitation sequencing (ChIP-seq), and to explore the relationship between H2BK120ub1 modification and gene expression in the neural tube development pathway, so as to provide a biological target for the diagnosis and treatment of NTDs. Methods The pregnant mice were randomly divided into control group and RA-induced NTDs group. DNA of samples was extracted by ChIP-seq. The compared reads were obtained, then peak analysis of whole genome, GO enrichment analysis of the biological functions of the related peak genes were conducted. Results Totally 306 genes displayed significant H2BK120ub1 differences between control group and NTDs group. The top three genes in the cellular component were Cell (82 genes), Cell part (82 genes), and Organelle part (52 genes). The top three genes in the biological process were celluar process (68 genes), metabolic process (47 genes) and biological regulation (40 genes). The results of KEGG pathway analysis were mainly enriched in differential genes involved in key signaling pathways that regulate long plate chondro-genesis such as hedgehog factor (HH) signaling pathway, TGF-beta signaling pathway and Wnt signaling pathway(P<0.05). Conclusion H2BK120ub1 may be a potential biomarker or a promising target for epigenetic-based NTDs disease treatment.

Key words

retinoic acid / H2BK120ub / neural tube defects / chromatin immunoprecipitation sequencing

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WANG Shan, HE Xue-jia, CHENG Xi-yue, LIN Ye, PEI Pei, ZHAN Xiao-jun. Genome-wide changes of H2BK120ub1 inmouse neural tube defect embryos induced by retinoic acid[J]. Chinese Journal of Child Health Care. 2022, 30(7): 731-736 https://doi.org/10.11852/zgetbjzz2021-1609

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