Objective To establish Chinese norm of General Physical Activity Scale for children aged 3 to 6, and to explore its cut-off score for the norm, thus to provide a practical tool for clinical application.Methods From August 2020 to May 2021, a stratified cluster sampling approach was utilized to recruit 1 616 children aged 3 to 6 years from eight cities in six major administrative regions of China. The norm distribution and scores were analyzed, and its cut-off score was discussed.Results The scores of each dimension was significantly different among eight cities(F=10.964 - 117.766, P<0.001). The area under the receiver operating characteristic curve of the total score of the scale is 0.937(95%CI:0.916 - 0.957). The maximum Yuden Index(YI) was 0.74, and the corresponding total score was in the 17th percentile(P₁₇) in the norm, with the sensitivity of 0.871 and specificity of 0.864. When P₁₅ (76)and P₂₀(80)were taken as the threshold point, the corresponding YI was 0.69 and 0.72, respectively, and the sensitivity and specificity were both above 0.8.Conclusions The norm sample of this study is representative, and the cut-off score is appropriate, which can be popularized and applied in clinic practice.