Influencing factors of γδ-T cells in peripheral blood and its predictive value for outcomes in premature infants

JIN Xin-yun; ZHANG Xiao-li; WANG Yin-juan; LIU Jia-xin; WANG Xiao-yang; XU Fa-lin

doi:10.11852/zgetbjzz2021-1011

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Chinese Journal of Child Health Care ›› 2022, Vol. 30 ›› Issue (10) : 1118-1122. DOI: 10.11852/zgetbjzz2021-1011

Clinical Research

Influencing factors of γδ-T cells in peripheral blood and its predictive value for outcomes in premature infants

JIN Xin-yun^*, ZHANG Xiao-li, WANG Yin-juan, LIU Jia-xin, WANG Xiao-yang, XU Fa-lin

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Abstract

Objective To investigate the influence of perinatal risk factors on γδ-T lymphocyte subsets in peripheral blood of preterm infants, and to determine the prognostic value of γδ-T cells for outcomes of premature infants. Methods A total of 112 preterm infants with gestational age from 28 to 37 weeks were enrolled in this study (including 88 cases of early preterm infants, 24 cases of middle to late preterm infants). Meanwhile 15 infants with gestational age of 37 to 42 weeks were selected into the control group. The levels of γδ-T, CD4+, CD8+, CD3+ and total lymphocytes in peripheral blood within 24 hours after birth were tested by flow cytometry. The influence of gestational age and risk factors of preterm birth on the lymphocyte subsets levels, as well as the relationship between outcomes of premature infants and γδ-T cells were analyzed. Results 1)There was significantly difference in the level of γδ-T cell in peripheral blood among the early preterm infants,middle to late preterm infants and term infants(F=8.855,P<0.001),and the proportion of γδ-T cells in early preterm infants was significantly lower than that in the other two groups(P＜0.05). 2) There was no significant difference in lymphocyte subsets among different causes of preterm birth groups (P＞0.05). 3)The levels of γδ-T cells in the four different iatrogenic preterm delivery subgroups were significantly different(F=3.595,P<0.05), andthe proportion of γδ-T cells in the fetal growth restriction group was significantly decreased (P<0.05). 4) There was no significant difference in the proportion of lymphocyte subsets between early-onset infection group and non-early-onset infection group (P>0.05). 5) No significant difference in γδ-T lymphocyte subsets was found among the normal group, the cerebral palsy/developmental delays group and the death group with 6 and 12 months of corrected age (P>0.05). Conclusion γδ-T cells in early peripheral blood of preterm infants are mainly affected by gestational age and are sensitive to fetal growth restriction, but have no correlation with the short-term development level of preterm infants.

Key words

preterm infants / gestational age / lymphocyte subsets / γδ-T cell / perinatal factors

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JIN Xin-yun, ZHANG Xiao-li, WANG Yin-juan, LIU Jia-xin, WANG Xiao-yang, XU Fa-lin. Influencing factors of γδ-T cells in peripheral blood and its predictive value for outcomes in premature infants[J]. Chinese Journal of Child Health Care. 2022, 30(10): 1118-1122 https://doi.org/10.11852/zgetbjzz2021-1011

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