Objective To explore the relationship between serum high mobility group box-1 protein (HMGB1), soluble cluster differentiation 163(sCD163) levels andfractional exhaled nitric oxide(FeNO) concentration and disease severity in children with bronchiolitis, and to analyze the diagnostic value of serum HMGB1 and sCD163 for bronchiolitis in children. Methods A total of 76 children with bronchiolitis for the first time in the First Affiliated Hospital of Shaoyang University from September 2018 to September 2019 were selected into the study group, including mild group(n=23), moderate group(n=31) and severe group (n=22).At the same time, 50 healthy children at the same age were selected into the control group.Serum levels of HMGB1 and sCD163 were detected by enzyme linked immunosorbent assay, FeNO concentration was measured by off-line tidal exhalation.Pearson method was used to analyze the correlation between serum HMGB1, sCD163 levels and FeNO concentration in children with bronchiolitis.Receiver operating characteristic curve(ROC) was used to evaluate the predictive value of serum HMGB1 and sCD163 levels in children with bronchiolitis. Results The levels of HMGB1 and sCD163 in the study group were significantly higher than those in the control group (t=8.927, 8.442, P<0.001).With the severity of bronchiolitis aggravating, the levels of serum HMGB1, sCD163 and FeNO became higher (F=67.908, 96.412, 23.390, P<0.001).Pearson results showed that serum HMGB1 and sCD163 levels were positively correlated with FeNO concentration in children with bronchiolitis (r=0.674,0.680, P<0.001).ROC results showed that the area under the curve (AUC) of serum HMGB1 and sCD163 in the diagnosis of bronchiolitis was 0.849 (95%CI:0.775 - 0.897) and 0.863 (95%CI:0.802—0.906), respectively.The AUC of combined diagnosis was 0.925 (95% CI:0.870 - 0.968).Serum HMGB1 and sCD163 levels were positively correlated with the number of wheezing during follow-up (r=0.614, 0.672, P<0.001). Conclusion The levels of serum HMGB1 and sCD163 in children with bronchiolitis are positively correlated with FeNO concentration and severity of disease, which may be used as potential biological indicators to predict the occurrence, development and prognosis of bronchiolitis.
Key words
children with bronchiolitis /
high mobility group box-1 protein(HMGB1) /
soluble cluster differentiation 163 /
fractional exhaled nitric oxide(FeNO) /
severity
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