Objective To explore the changes of serum cystatin C (CysC) and humoral immune function indexes in asthma children and their correlation with Mycoplasma pneumoniae (MP) infection, in order to provide reference for the treatment of asthma. Methods A total of 95 asthma children treated in Suzhou Ninth People's Hospital and Wujiang District Children's Hospital from September 2017 to June 2020 were enrolled into study group, meanwhile 63 children who took physical examination were selected as control group. Serum levels of CysC, humoral immune function indexes [immunoglobulin A (IgA), immunoglobulin G (IgG), immunoglobulin M (IgM)] and MP infection rate were compared between the study group and control group. According to the severity of asthma, chidlren were divided into severe group and mild group. Serum CysC level, humoral immune function indexes and MP infection rate were compared. The correlation between MP infection and CysC level, humoral immune function indexes was analyzed. The evaluation value of each index and their combination for asthma severity was analyzed. The lung function indexes of all subjects were detected. According to MP infection, they were divided into MP infection group and non-infection group. The lung function indexes were compared between the two groups and control group. Results The levels of CysC and IgM, and MP infection rate in study group were higher than those in control group(t=9.942,2.880, χ2=10.914, P<0.05), while levels of IgA and IgG in study group were significantly lower than those in control group (t=10.289, 7.968, P<0.05). CysC and IgM levels were positively correlated with MP infection(r=0.170, 0.183, P<0.05), while IgG level was negatively related to MP infection (r＝-0.254, P<0.05). The levels of CysC and IgM, and MP infection rate in severe group were higher than those in mild group (t=3.695, 2.090, χ2 =18.459, P<0.05), while levels of IgA and IgG in severe group were significantly lower than those in mild group (t=3.016, 3.192, P<0.05). The diagnostic value of serum CysC level combined with humoral immune function indexes and MP infection for predicting asthma severity was significantly higher than that of single index detection (Z=2.018, 2.899, 2.068, 3.094, 2.799, P<0.05). The forced vital capacity (FVC), forced expiratory volume in one second (FEV1) and FEV1/FVC in MP infection group were lower than those in non-infection group (t=2.437, 2.597, 2.261, P<0.05). Conclusions The serum CysC level and humoral immune function are abnormal in asthma children. The combined detection of serum CysC level, humoral immune function indexes and MP infection is of diagnostic value for asthma severity.
Key words
child asthma /
cystatin C /
humoral immunity /
mycoplasma pneumoniae
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