Objective To detect the expression levels of microRNA-138 (miR-138) and microRNA-338 (miR-338) in children with spastic cerebral palsy and explore the clinical value. Methods A total of 64 children with spastic cerebral palsy who were treated in the Third Affiliated Hospital of Zhengzhou University from March 2018 to April 2020 were selected into spastic cerebral palsy group. Meanwhile,62 healthy children were selected as healthy control group. The expression levels of miR-138 and miR-338 were detected by real-time quantitative PCR (qRT-PCR),the serum levels of tumor necrosis factor-α (TNF-α),C-reactive protein (CRP),nerve growth factor (NGF) and endothelin (ET) were detected by enzyme-linked immunosorbent assay (ELISA). Pearson method was used to analyze the correlation between serum miR-138,miR-338 and TNF-α,CRP,NGF,ET expression levels in children with spastic cerebral palsy Receiver operating characteristic curve (ROC) was used to analyze the diagnostic value of serum miR-138 and miR-338 in spastic cerebral palsy. Multivariate Logistic regression analysis was used to analyze the influencing factors of spastic cerebral palsy. Results The expression levels of miR-138,miR-338,TNF-α,CRP and ET in serum in spastic cerebral palsy group were higher than those in healthy control group,while NGF expression level was lower than that in control group(t=9.598,9.592,11.039,42.397,14.172,5.744,P＜0.05). There was a positive correlation between serum miR-138 and miR-338 in children with spastic cerebral palsy (r = 0.687,P < 0.05),and they were positively correlated with TNF-α,CRP,ET levels (miR-138:r=0.519,0.483,0.494;miR-338:r=0.506,0.492,0.516,P＜0.05),and negatively correlated with NGF level (r=-0.522,-0.496,P＜0.05). The area under the curve (AUC) of serum miR-138 and miR-338 in the diagnosis of spastic cerebral palsy was 0.882 and 0.858,the cut-off value was 1.189 and 1.406,the specificity was 75.8%,93.5%,and the sensitivity was 87.5% and 67.2%,respectively.The AUC of the combined diagnosis was 0.950,the specificity was 90.3%,and the sensitivity was 93.8%. Logistic regression analysis that miR-138,miR-338 and NGF were protective factors of spastic cerebral palsy [OR (95%CI):2.280 (1.742-2.983),2.222 (1.637-3.016),P<0.05],and NGF was risk factor of spastic cerebral palsy [OR (95%CI):0.720 (0.527-0.984),P＜0.05]. Conclusions The serum expression levels of miR-138 and miR-338 in children with spastic cerebral palsy are significantly up-regulated,and they may play an important role in the occurrence and development of spastic cerebral palsy.
Key words
spastic cerebral palsy /
serum /
microRNA-138 /
microRNA-338 /
children
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] Fiss AL,Jeffries L,Yocum A,et al. Validity of the early activity scale for endurance and the 6-minute walk test for children with cerebral palsy[J]. Pediatr Phys Ther,2019,31(2):156-163.
[2] 刘芳,杜成芬. 中医干预联合神经生长因子治疗痉挛型脑瘫患儿疗效及对脑CT及SPECT的影响[J]. 现代中西医结合杂志,2018,27(2):165-168.
[3] Kruse A,Schranz C,Tilp M,et al. Muscle and tendon morphology alterations in children and adolescents with mild forms of spastic cerebral palsy[J]. BMC Pediatr,2018,18(1):156-170.
[4] Ji H,Xu LH,Wang Z,et al. Differential microRNA expression in the prefrontal cortex of mouse offspring induced by glyphosate exposure during pregnancy and lactation[J]. Exp Ther Med,2018,15(3):2457-2467.
[5] 袁红刚,李三梅. MiR-138在脑胶质瘤患者血清表达的生物学效应及临床意义[J]. 转化医学杂志,2020,9(1):4-7.
[6] 郭金鼎,赵新,劳杰,等. miR-124和miR-138参与调控健侧颈7移位术后脑功能重塑的研究[J]. 中华手外科杂志,2019,35(2):139-142.
[7] 吴大英,黄震,张秀艳,等. MiR-338及PTN的表达与卵巢癌的临床诊断价值的研究[J]. 医学理论与实践,2020,33(17):2798-2801.
[8] Birch D,Britt BC,Dukes SC,et al. MicroRNAs participate in the murine oligodendroglial response to perinatal hypoxia-ischemia[J]. Pediatr Res,2014,76(4):334-340.
[9] 李晓捷,唐久来,马丙祥,等. 脑性瘫痪的定义、诊断标准及临床分型[J]. 中华实用儿科临床杂志,2014,29(19):1520-1520.
[10] Saussez G,Van-Laethem M,Bleyenheuft Y.Changes in tactile function during intensive bimanual training in children with unilateral spastic cerebral palsy[J]. J Child Neurol,2018,33(4):260-268.
[11] Kuchen DB,Eichelberger P,Baur H,et al. Long-term follow-up after patellar tendon shorteningfor flexed knee gait in bilateral spastic cerebral palsy[J]. Gait Posture,2020,81(1):85-90.
[12] Kara OK,Yardimci BN,Livanelioglu A,et al. Examination of touch-coordinate errors of adolescents with unilateral spastic cerebral palsy at an aiming-tapping task[J]. J Back Musculoskelet Rehabil,2020,33(1):81-89.
[13] 王军,郎永斌,杜江华,等. 悬吊运动训练对痉挛型脑瘫患儿运动及平衡功能的影响[J]. 中国当代儿科杂志,2018,20(6):38-42.
[14] Tseng AM,Chung DD,Pinson MR,et al. Ethanol exposure increases miR-140 in extracellular vesicles:Implications for fetal neural stem cell proliferation and maturation[J]. Alcohol Clin Exp Res,2019,43(7):1414-1426.
[15] Yang J,Xiong L,Wang Y,et al. Oligodendrocyte precursor cell transplantation promotes functional recovery following contusive spinal cord injury in rats and is associated with altered microRNA expression[J]. Mol Med Rep,2018,17(1):771-782.
[16] 李会兵,姚娟. miR-138靶向作用Sema4C对脑胶质瘤细胞侵袭转移的影响[J]. 国际肿瘤学杂志,2018,45(3):139-142.
[17] Nazari B,Soleimanifar F,Kazemi M,et al. Derivation of preoligodendrocytes from human-induced pluripotent stem cells through overexpression of microRNA 338[J]. J Cell Biochem,2019,120(6):9700-9708.
[18] 李海霞,覃蓉,刘娟,等. 健脑益智合剂对脑瘫幼鼠模型脑组织病理学及TNF-α表达的影响[J]. 中医药导报,2019,25(22):36-38.
[19] Dick AG,Magill N,White TCH,et al. C-reactive protein:what to expect after bony hip surgery for nonambulatory childrenand adolescents with cerebral palsy[J]. J Pediatr Orthop B,2019,28(4):309-313.
[20] 张鹤,白青云,党伟利,等. 针刺俞募穴辅助治疗小儿脑瘫合并反复呼吸道感染临床研究[J]. 新中医,2019,51(2):223-226.
[21] Masuoka T,Yamashita Y,Yoshida J,et al. Sensitization of glutamate receptor-mediated pain behavior via nerve growth factor-dependent phosphorylation of transient receptor potential V1 under inflammatory conditions[J]. Br J Pharmacol,2020,177(18):4223-4241.
[22] 吴蔚然,周厚地,刘娟. 芪丹通络颗粒联合硫辛酸治疗糖尿病周围神经病变的临床研究[J]. 现代药物与临床,2020,35(2):267-270.
PDF(710 KB)
