Objective To investigate the relationship between polymorphism of leukotriene C4 synthase (LTC4S) gene rs730012 and susceptibility of respiratory syncytial virus (RSV) bronchiolitis and the efficacy of montelukast sodium. Methods A total of 120 children diagnosed with RSV bronchiolitis in Nanyang Second General Hospital were selected as case group,and 80 healthy children were selected as control group. Genotypes of LTC4S rs730012 in two groups were detected. The case group was treated with Montelulast sodium orally for 2 weeks based on the routine treatment. The differences in clinical manifestations of children in the case group with different genotypes were analyzed,and the changes in serum cysteamine leukotriene (CysLTs),urinary leukotriene E4(LTE4),eosinophi cationic protein (ECP) levels and moisture respiratory pulmonary function parameters were detected before and after treatment. Results The distribution of genotype and allele frequency of LTC4S rs730012 showed no significant difference between the case group and control group (χ2=0.797,0.624,P>0.05). After treatment,the clinical symptom score after one week,the duration of cough,breathing and wheezing and the hospitalization days in AC/CC group were significantly lower than those in AA group(t=3.552,2.284,3.446,2.716,2.871,P<0.05).After one week of treatment,the levels of serum CysLTs,urine LTE4 and ECP in the AC/CC group were significantly lower than those in AA group(t=4.653,5.917,9.933,P<0.001).Additionally, the lung function indexes like tidal volume,TPTEE/TE and VPEE/VE were also significantly higher than those of AA group(t=2.090,2.104,2.312,P<0.05). Conclusions There was no correlation between LTC4S gene polymorphism and susceptibility in children with RSV bronchiolitis,but it may be related to the efficacy of montelulast sodium. The efficacy of RSV children with AC/CC genotype is relatively better than that of RSV children with AA genotype.
Key words
bronchiolitis /
leukotriene C4 synthase /
polymorphism /
montelukast sodium
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