Objective To analyze the characteristics of structural changes of intestinal flora in infants with neonatal respiratory distress syndrome (NRDS), in order to provide theoretical basis for the clinical application of probiotics for NRDS assisted therapy. Methods A total of 15 premature infants with NRDS who were hospitalized in the Neonatology Department of Jinan Central Hospital from January to May 2020 were selected into the NRDS group in this study. Meanwhile, 15 non-NRDS hospitalized premature infants were selected as the control group.The clinical data, blood sample, stool sample of the participants were collected. The stool DNA was extracted, high-throughput 16S rRNA gene sequencing was conducted, and bioinformatics analysis was conducted to compare the differences of intestinal flora between the two groups. Results 1) Compared with the control group, the OTU number of intestinal flora in the NRDS group was lower in each level of bacteria classification, including species, genus, family, order, class and phylum.2) The β diversity analysis showed that there were significant differences in intestinal flora structure between NRDS group and control group. 3) At the level of "family", Enterobacteriaceae was enriched in the NRDS group (Z=2.78, P=0.009). At the level of "phylum", Firmicutes were enriched in the control group (Z=2.18, P=0.021) and Proteobacteria were enriched in the NRDS group (Z=2.02,P=0.014). Conclusions The ecological imbalance of intestinal flora is found in NRDS infants, and the richness and diversity of flora species are decreasing. The structural distribution of intestinal flora in infants with NRDS is significantly different from that of normal preterm infants.
Key words
neonatal respiratory distress syndrome /
gut microbiota /
short-chain fatty acids /
16S ribosomal RNA
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References
[1] Sweet DG,Carnielli V, Greisen G, et al. European Q consensus guidelines on the management of respiratory distress syndrome - 2019 update[J]. Neonatology,2019,115(4):432-450.
[2] Marseglia L, D′Angelo G, Granese R, et al. Role of oxidative stress in neonatal respiratory distress syndrome[J]. Free Radic Biol Med,2019,142:132-137.
[3] Tirone C, Pezza L, Paladini A, et al. Gut and lung microbiota in preterm infants: immunological modulation and implication in neonatal outcomes[J]. Front Immunol,2019,10:2910.
[4] Arpaia N, Campbell C, Fan X ,et al. Metabolites produced by commensalbacteria promote peripheral regulatory T-cell generation[J]. Nature,2013,504(7480):451-455.
[5] Atarashi K, Tanoue T, Oshima K,et al. Treg induction by a rationally selected mixture of clostridia strains from the human microbiota[J]. Nature,2013,500:232-236.
[6] Oh KJ, Park JY, Lee J, et al. The combined exposure to intra-amniotic inflammation and neonatal respiratory distress syndrome increases the risk ofintraventricular hemorrhage in preterm neonates[J]. J Perinat Med,2018,46:9-20.
[7] Zhang C, Zhu X. Clinical effects of pulmonary surfactant in combination with nasal continuous positive airway pressure therapy on neonatal respiratory distress syndrome[J]. Pak J Med Sci, 2017,33:621-625.
[8] Sweet DG,Carnielli V, Greisen G, et al. European consensus guidelines on the management of respiratory distress syndrome [J]. Neonatology, 2017,111(2):107-125.
[9] Krautkramer KA,Fan J,Bckhed F.Gut microbial metabolites as multi-kingdom intermediates[J].Nature Reviews Microbiology,2020. doi:1038/s41579-020-0438-4.
[10] Jobe A, Ikegami M, Glatz T, et al. Saturated phosphatidylcholine secretion and the effect of natural surfactant on premature and term lambs ventilated for 2 days[J]. Exp Lung Res, 1983, 4(4):259-267.
[11] Bohlin K, Merchak A, Spence K, et al. Endogenous surfactant metabolism in newborn infants with and without respiratory failure[J]. Pediatric Res, 2003, 54(2):185-191.
[12] Nicolas GR, Chang PV. Deciphering the chemical lexicon of host–gutmicrobiota interactions[J].Trends Pharmacol Sci,2019,40(6):430-445.
[13] Wang S, Egan M, Ryan CA, et al. A good start in life is important-perinatal factors dictate earlymicrobiota development and longer term maturation[J]. FEMS Microbiol Rev,2020,44(6):763-781.
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