Objective To design the hour-specific bilirubin nomogram of Han newborns in the first 120 hours after birth, and to compare the difference in the trends of the hour-specific bilirubin and the incidence of hyperbilirubinemia between newborns in Aksu and Yibin, so as to provide evidence for the prevention, assessment and treatment of hyperbilirubinemia. Method From January 2018 to December 2019, healthy Han infants were selected in this study, who were born in the obstetrics department of the Third People's Hospital of Yibin and the obstetrics department of the Corps First Division Hospital's of Aksu. The related research indexes that may be related to neonatal jaundice were recorded detailedly. The transcutaneous bilirubin(TCB) value of 5 days after birth was monitored. TCB percentiles(P₂₅,P₅₀,P₇₅) of each period were calculated and plotted. At the same time,the influence of related perinatal factors on neonatal hyperbilirubinemia was statistically analyzed. Results A total of 1 605 cases were included in this study. The TCB percentiles of the Han neonates in Yibin area were higher than those in Aksu at same time points. The incidence of hyperbilirubinemia of Han neonates in Yibin was higher than that of Aksu(χ²=118.5,P<0.001). Premature, first feeding time>24 h,the application of oxytocin in pregnant women and cephalohematoma were risk factors of hyperbilirnbinemia(OR=8.331,19.640,2.982,4.019). Conclusions The bilirubin level of neonates increases with the growth of age within 5 days after birth,and the rising rate of hour-specific bilirubin of Yibin neonates is higher than that of Aksu neonates.The incidence of hyperbilirubinemia of Yibin neonates is also higher than that of Aksu neonates.