Objective To analyze the changes and correlation of serum 25-hydroxyvitamin D3 [25-(OH)D3], the number of T lymphocytes and related cytokines in children with asthma at different stages, and to explore the possible mechanism of 25-(OH)D3 involved in the pathogenesis and progression of asthma. Methods From January 2018 to December 2019, a total of 105 children with asthma and 30 healthy children in Sichuan Provincial People's Hospital were selected into this study, including 25 cases of acute attack, 40 cases of chronic duration and 40 cases of remission.The level of 25-(OH)D3 were detected by electrochemical stripping voltammetry and the levels of T lymphocyte subsets and their cytokines IFN- γ, IL-4 and IL-10 were detected by flow cytometry. Results In children with asthma, serum 25-(OH)D3 level decreased significantly, the number of CD3, CD4/CD8 cells as well as the secretion level of IFN-γ and IL-10 decreased, while the level of IL-4 secretion increased.Changes were most pronounced during acute episodes, followed by chronic persistence, and closest to normal during remission.The level of serum 25-(OH) D3 in children with asthma at different stages was positively correlated with the level of IFN-γ and IL-10, and negatively correlated with the level of IL-4. Conclusions Vitamin D, as an immunomodulatory hormone, is closely related to the onset and disease control of asthma.Monitoring serum 25-(OH) D3 level of asthmatic patients is helpful for the judgment and the treatment of the disease condition, providing a new monitoring method for the clinical practice.
Key words
childhood asthma /
vitamin D /
T cell subsets /
cytokines
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References
[1] 中华医学会儿科学分会呼吸学组.儿童支气管哮喘诊断与防治指南[J].中华儿科杂志,2016,54(3):167-181.
[2] Holgate ST.Innate and adaptive immune responses in asthma[J]. Nat Med,2012,18(5):673-683.
[3] 李敏,宋丽,张剑波,等.哮喘患儿CD4+CD25+调节性T细胞及IL-10和TGF-β1的变化[J].中国当代儿科杂志,2009,11(10):829-832.
[4] Raby BA,Lazarus R,Silverman E,et al.Association of vitamin D receptor gene polymorphisms with childhood and adult asthma[J]. Am J Respir Crit Care Med,2004,170(10):1057-1065.
[5] 李飞,高金明.维生素D、维生素D受体与支气管哮喘[EB/J].中华哮喘杂志(电子版),2010,4(1):38-42.
[6] 修小莹.维生素D与儿童支气管哮喘的关系[J].贵州医药,2018,42(2):167-169.
[7] 王学亮,周传麟,王丽娜,等.维生素D对支气管哮喘患者Th1/Th2平衡的调节作用及IL-17的影响[J].国际呼吸杂志,2015,35(1):9-11.
[8] Chinellato I,Piazza M, Sandri M,et al. Vitamin D serum levels and markers of asthma control in Italian children[J].J Pediatr,2011,158(3):437-441.
[9] Bener A,Ehlayel MS, Tulic MK,et al.Vitamin D deficiency as a strong predictor of asthma in children[J].Int Arch Allergy Immunol, 2012,157(2):168-175.
[10] Babar MZM,Hussain M,Majeed SA.Vitamin D supplementation improves FEV1 in patients of bronchial asthma[J].Pak J Med Sci,2017,33(5):1144-1147.
[11] 李如霞,侯进飞,周瑾思,等.1,25-二羟维生素 D3对尘螨引起的P815肥大细胞TLR4表达和IL-4分泌影响的初步研究[J].中华微生物学和免疫学杂志,2015,5(3):188-193.
[12] 万军营,张铁栓.补充维生素D对哮喘患者气道炎症反应及免疫功能的影响[J].中国临床研究,2019,32(1):23-26.
[13] 宋颖芳,赖国祥,柳德灵,等.1,25-二羟维生素 D3 负性调控被动致敏人气道平滑肌细胞中的 NF-κB 信号通路[J].中国病理生理杂志,2013,29(11):2044-2048.
[14] 胡翠萍.维生素D对哮喘儿童Th1/Th2/Th17/Treg免疫失衡的影响研究[J].中国预防医学杂志.2019,20(4):357-360.
[15] Ramos-Martineza E,Lopez-Vancella MR,Fernandez de Cordova Aguirreb JC,et al.Reduction of respiratory infections in asthma patients supplemented with vitamin D is related to increased serum IL-10 and IFNy levels and cathelicidin expression[J].Cytokine,2018,108:39-24.
[16] 王学亮,周传麟,王丽娜,等.维生素D对支气管哮喘患者Th2/Th2平衡的调节作用及IL-17的影响[J].国际呼吸杂志,2015,35(1):9-11.
[17] Khoo AL, Joosten I, Michels M, et al.1,25-Dihydroxyvitamin D3 inhibits proliferation but not the suppressive function ofregulatory T cells in the absence of antigen-presenting cells[J].Immunology, 2011, 134(4):459-468.
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