Objective To analyze the impacts of polyinfection on clinical characteristics of children with acute lower respiratory tract infection due to hBoV, and to analyze the high risk factors of the polyinfection. Method From November 2017 to October 2019, totally 3 350 samples of nasopharyngeal aspirates were collected for respiratory tract multi-pathogen detection from children with lower respiratory tract infection in the Respiratory Department of Children's Hospital Affiliated to Soochow University. Results Among the 3 350 samples, hBoV was found positive in 167 samples (5.0%), of which 102 (61.1%) were hBoV mono-infected and 65 (38.9%) poly-infected.In the polyinfected samples, the main pathogens included the virus (26, 15.6%), mycoplasma pneumonia (16, 9.6%) and bacteria (11, 6.6%), 12 samples (7.2%) were poly-infected with two or more pathogens.In terms of cough, wheezing, fever, dyspnea, lung rales and hospitalization stay, there was no statistically significant difference among the groups of mono-hBoV infected samples, poly-virus infected samples, poly-mycoplasma infected samples, poly-bacteria infected samples, and poly-infected samples with two or more of the pathogens(P＞0.05).The proportion of severe pneumonia in poly-bacteria infected samples was significantly higher than that in poly-virus and poly-mycoplasma infected samples(χ2=15.648, P<0.05).Logistic regression analysis indicated that pre-admission course exceeded 7 days was a risk factor for hBoV polyinfection (OR=1.040, 95%CI:1.002-1.079). Conclusions hBoV has a higher polyinfection rate.AND hBoV poly-bacterial infection may aggravate the disease.Children with pre-admission course ＞7 days should be alerted for hBoV polyinfection.
Key words
children /
hBoV /
polyinfection /
high risk factor
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References
[1] Allander T, Tamrm MT, Efiksson M, et al.Cloning of a human parvovirus by malecatar screening of respiratory tract samples[J].Proc Natl Acad Sci U S A,2005,102(36):12891-12896.
[2] Silva PE, Figueiredo CA, Luchs A, et al.Human bocavirus in hospitalized children under 5 years with acute respiratoryinfection, SāoPaulo, Brazil,2010[J].Arch Virol,2018,163(5):1325-1330.
[3] Verbeke V, Reynders M, Floré K,et al.Human bocavirus infection in belgian children with respiratory tract disease[J].Arch Virol, 2019,164(12):2919-2930.
[4] Ljubin-Sternak S, Meštrovic＇ T, Ivkovic＇ -Jurekovic＇ I,et al.High detection rates of human bocavirus in infants and small children with lower respiratory tract infection from croatia[J].Clin Lab, 2019,65(1).doi:10.7754.
[5] Moreno B, Abrego L,Carrera JP, et al.Carrera detection of human bocavirus type 1 infection in panamanian children with respiratory illness[J].J MedVirol,2016,88(3):389-394.
[6] 王丽,黄娟,莫庆仪,等.中山地区住院患儿呼吸道博卡病毒感染的临床研究[J].检验医学与临床,2019,16(8):1009-1014.
[7] 于乐香,陈正荣,蒋吴君,等.苏州地区儿童急性下呼吸道人鼻病毒感染的流行病学及喘息相关因素[J].中华实用儿科临床杂志,2015,30(23):1816-1820.
[8] Petrarca L, Nenna R, Frassanito A, et al.HumanBocavirus in children hospitalized for acute respiratory tract infection in Rome[J].World J Pediatr, 2019,27(11):1-6.
[9] Calvo C, García-García ML, Pozo F, et al.Respiratory syncytial virus coinfections with rhinovirus and human bocavirus in hospitalized children[J].Medicine, 2015, 94(42):e1788.
[10] Sun H, Sun J, Ji W, et al.Impact of RSV coinfection on human bocavirus in children with acute respiratory infections[J].J Trop Pediatr, 2019, 65(4):342-351.
[11] 刘晓红,谢正德,任丽丽,等.儿童急性下呼吸道人博卡病毒感染及临床特征的比较研究[J].中国病毒病杂志,2017,7(6):425-430.
[12] 门玉梅,李文秀,樊妮,等.重症人类博卡病毒肺炎住院患儿混合感染的临床研究[J].中华实用儿科临床杂志,2019,34(14):1097-1099.
[13] Zhou J, Peng Y, Peng X, et al.Human bocavirus and human metapneumovirus in hospitalized children with lower respiratory tract illness in Changsha, China[J].Influenza Other Respir Viruses, 2018, 12(2):279-286.
[14] Principi N, Piralla A, Zampiero A, et al.Bocavirus infection in otherwise healthy children with respiratory disease[J].PLoS ONE,2015,10(8):e0135640.
[15] 丁小芳,张兵,钟礼立,等.人博卡病毒载量与儿童急性下呼吸道感染临床特征相关性研究[J].中国当代儿科杂志,2017,19(3):327-330.
[16] Deng Y, Gu X, Zhao X,et al.High viral load of human bocavirus correlates with duration of wheezing in children with severe lower respiratory tract infection[J].PLoS ONE,2012,7(3):e34353.
[17] Jiang W, Yin F,Zhou W,et al.Clinical significance of different virus load of human bocavirus in patients with lower respiratory tract infection[J].Sci Rep, 2016,6:20246.doi:10.1038/srep20246.
[18] 何香萍,王宇清.苏州地区儿童呼吸道人类博卡病毒混合感染临床研究[J].儿科药学杂志,2017,23(12):7-9.
[19] Dapalma T, Doonan BP, Trager NM, et al.A systematic approach to virus-virus interactions[J].Virus Res,2010,149(1):1-9.
[20] Verbeke V,Reynders M,Floré K,et al.Human bocavirus infection in Belgian children with respiratory tract disease[J].Arch Virol,2019,164(12):2919-2930.
[21] Xu M, Arku B, Jartti T,et al.Comparative diagnosis of human bocavirus 1 respiratory infection with messenger rna reverse-transcription polymerase chain reaction (PCR), DNA quantitative PCR, and serology[J].J Infect Dis, 2017,215(10):1551-1557.
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