Investigation on the changes of intestinal flora in children with cerebral palsy using high-throughput sequencing

ZHOU Bo-lan; HUANG Cong-fu; PENG Yuan-ping; LI Yin-hu; LI Xiu-yun; WANG Pei-qin; WU Gen-feng; JIAO Guang

doi:10.11852/zgetbjzz2019-1418

PDF(1508 KB)

Chinese Journal of Child Health Care ›› 2020, Vol. 28 ›› Issue (10) : 1077-1082. DOI: 10.11852/zgetbjzz2019-1418

Investigation on the changes of intestinal flora in children with cerebral palsy using high-throughput sequencing

ZHOU Bo-lan¹, HUANG Cong-fu¹, PENG Yuan-ping², LI Yin-hu³, LI Xiu-yun¹, WANG Pei-qin¹, WU Gen-feng¹, JIAO Guang⁴

Author information +

History +

Abstract

Objective To explore the changes of intestinal flora in children with cerebral palsy by high-throughput sequencing. Methods Thirty-four children with cerebral palsy diagnosed in Longgang District Social Welfare Center and eating normally were selected as the cerebral palsy group,and 14 children with cerebral palsy accompanied by epilepsy and eating normally were selected as the cerebral palsy accompanied by epilepsy group,meanwhile 22 healthy children were selected as the healthy group.Total DNA was extracted and amplified from fecal specimens of all subjects.The 16SrRNA gene was sequenced with high throughput and the sequencing results were analyzed biologically. Results 1) The intestinal flora structure of healthy group was significantly different from that of cerebral palsy group and cerebral palsy accompanied by epilepsy group (R²=0.410,P=0.001;R²=0.450,P=0.001),but the intestinal flora structure of cerebral palsy group was similar to that of cerebral palsy accompanied by epilepsy group (R²=0.025,P=0.272).2) The abundance of Prevotella in children with cerebral palsy and cerebral palsy increased significantly (32.68% in cerebral palsy group and 34.34% in cerebral palsy accompanied by epilepsy group). Conclusions Prevotella abundance increases significantly in children with cerebral palsy and cerebral palsy accompanied by epilepsy who ate normally.Prevotella may be a protective bacteria of children with cerebral palsy who ate normally.

Key words

high-throughput sequencing / cerebral palsy / intestinal flora

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations

ZHOU Bo-lan, HUANG Cong-fu, PENG Yuan-ping, LI Yin-hu, LI Xiu-yun, WANG Pei-qin, WU Gen-feng, JIAO Guang. Investigation on the changes of intestinal flora in children with cerebral palsy using high-throughput sequencing[J]. Chinese Journal of Child Health Care. 2020, 28(10): 1077-1082 https://doi.org/10.11852/zgetbjzz2019-1418

References

[1] 中国康复医学会儿童康复专业委员会,中国残疾人康复协会小儿脑性瘫痪康复专业委员会,《中国脑性瘫痪康复指南》编委会.中国脑性瘫痪康复指南(2015):第一部分[J].中国康复医学杂志,2015,30(7):747-753.
[2] 王姣姣,张瑞光,董晓荣.肠道菌群与大脑功能相互联系的研究进展[J].中华神经医学杂志,2018,17(12):1281-1286.
[3] 黄从付,彭远平,李寅虎,等.脑瘫伴癫痫儿童肠道菌群变化[J].中国微生态学杂志,2019,31(4):402-406.
[4] Huang CF,Li YH,Li DF,et al.Distinct gut microbiota composition and functional category in children with cerebral palsy and epilepsy[J].Front Pediatr,2019,7:394.
[5] Wang Y,Kasper LH.The role of microbiome in central nervous system disorders[J].Brain Behav Immun,2014,38:1-12.
[6] Tlaskalova-Hogenova H,Stepankova R,Kozakova H,et al.The role of gut microbiota (commensal bacteria) and the mucosal barrier in the pathogenesis of inflammatory and autoimmune diseases and cancer:contribution of germ-free and gnotobiotic animal models of human diseases[J].Cell Mol Immunol,2011,8(2):110-120.
[7] Galland L.The gut microbiome and the brain[J].J Med Food,2014,17(12):1261-1272.
[8] De Filippis F,Pellegrini N,Laghi L,et al.Unusual sub-genus associations of faecal prevotella and bacteroides with specific dietary patterns[J].Microbiome,2016,4(1):57.
[9] De Filippo C,Cavalieri D,Di Paola M,et al.Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa[J].Proc Natl Acad Sci USA,2010,107:14691-14696.
[10] Arumugam M,Raes J,Pelletier E,et al.Enterotypes of the human gut microbiome[J].Nature,2011,473:174-180.
[11] Kang DW,Park JG,Ilhan ZE,et al.Reduced incidence of Prevotella and other fermenters in intestinal microflora of autistic children[J].PLoS One,2013,8(7):e68322.
[12] Huws SA,Kim EJ,Lee MRF,et al.As yet uncultured bacteria phylogenetically classified as Prevotella,Lachnospiraceaeincertaesedis and unclassified Bacteroidales,Clostridiales and Ruminococcaceae may play apredominant role in ruminalbiohydrogenation[J].Environ Microbiol,2011,13:1500-1512.
[13] Dyall SC.Interplay Between n-3 and n-6 long-chain polyunsaturated fatty acids and the endocannabinoid system in brain protection and repair[J].Lipids,2017,52(11):885-900.
[14] Tremlett H,Bauer KC,Appel-Cresswell S,et al.The gut microbiome in human neurological disease[J].Ann Neurol,2017,81(3):369-382.
[15] Tao J H,Duan J A,Jiang S,et al.Simultaneous determination of six short-chain fatty acids in colonic contents of colitis mice after oral administration of polysaccharides from chrysanthemum morifolium Ramat by gas chrom-atography with flame ionization detector[J].J Chromatogr B Analyt Technol Biomed Life Sci,2016,1029-1030:88-94.
[16] Barcelo A,Claustre J,Moro F,et al.Mucin secretion is modulated by luminal factors in the isolated vascularly perfused rat colon[J].Gut,2000,46(2):218-224.
[17] Goverse G,Molenaar R,Macia L,et al.Diet-derived short chain fatty acids stimulate intestinal epithelial cells to induce mucosal tolerogenic dendritic cells[J].J Immunol,2017,198(5):2172-2181.
[18] Rooks MG,Garrett WS.Gut microbiota,metabolites and host immunity[J].Nat Rev Immunol,2016,16(6):341-352.
[19] Nastasi C,Candela M,Bonefeld C M,et al.The effect of short-chain fatty acids on human monocyte-derived dendritic cells[J].Sci Rep,2015,5:16148.
[20] Jiang SY,Wei CC,Shang TT,et al.High glucose induces inflammatory cytokine through protein kinase C-induced toll-like receptor 2 pathway in gingival fibroblasts [J].Biochem Biophys Res Commun,2012,427(3):666-670.
[21] Vieira A T,Macia L,Galvao I,et al.A role for gut microbiota and the metabolite-sensing receptor GPR43 in a murine model of gout[J].Arthritis Rheumatol,2015,67(6):1646-1656.
[22] Wu GD,Chen J,Hoffmann C,et al.Linking long-term dietary patterns with gut microbial enterotypes[J].Science,2011,334(6052):105-108.
[23] Kovatcheva-datchary P,Nilsson A,Akrami R,et al.Dietary fiber-induced improvement in glucose metabolism is associated with increased abundance of prevotella[J].Cell Metab,2015,22(6):971-982.
[24] Mengxiang Z,Wei M,Juan Z,et al.Analysis of gut microbiota profiles and microbe-disease associations in children with autism spectrum disorders in China[J].Sci Rep,2018,8:13981.
[25] Parthasarathy G,Chen J,Chen X,et al.Relationship between microbiota of the colonic mucosa vs feces and symptoms,colonic transit,and methane production in female patients with chronic constipation[J].Gastroenterology,2016,150(2):367-379.
[26] Khan TJ,Ahmed YM,Zamzami MA,et al.Atorvastation treatment modulates the gut microbiota of the hyperchol-esterolemic patients[J].Omics,2018,22(2):154-163.
[27] Gomez-arango LF,Barrett HL,Mcintyre HD,et al.Connections between the gut microbiome and metabolic hormones in early pregnancy in overweight and obese women[J].Diabetes,2016,65(8):2214-2223.
[28] Kverka M,et al.Oral administration of Parabacteroides distasonis antigens attenuates experimental murine colitis through modulation of immunity and microbiota composition[J].Clin Exp Immunol,2011,163(2):250-259.
[29] Arpaia N,et al.Metabolites produced by commensal bacteria promote peripheral regulatory T-cell generation[J].Nature,2013,504(7480):451-455.
[30] Sears CL.In celebration of Sydney M.Finegold,M.D.:bacteroides fragilis in the colon:the good & the bad[J].Anaerobe,2012,18(2):192-196.
[31] Rehman A,Rausch P,Wang J,et al.Geographical patterns of the standing and active human gut microbiome in health and IBD[J].Gut,2016,65(2):238-248.
[32] Li YN,Huang F,Cheng HJ,et al.Intestine-derived Clostridium leptum induces murine tolerogenic dendritic cells and regulatory T cells in vitro[J].Hum Immunol,2014,75(12):1232-1238.
[33] Clavel T,Gomes-neto JC,Lagkouvardos I,et a1.Deciphering interactions between the gut microbiota and the immunesystem via microbial cultivation and minimal microbiomes[J].Immunol Rev,2017,279(1):8-22.
[34] Zhang J,Guo Z,Xue Z,et al.A phylo-functional core of gut microbiota in healthy young Chinese cohorts across lifestyles[J].ISME J,2015,9:1979-1990.
[35] Weiguang C,Fanlong L,Zongxin L,et al.Human intestinal lumen and mucosa-associated microbiota in patients with colorectal cancer[J].PLoS One,2012,7(6):e39743.
[36] Quitadamo P,Thapar N,Staiano A,et al.Gastrointestinal and nutritional problems in neurologically impaired children [J].Eur J Paediatr Neurol,2016,20(6):810-815.