Objective To analyze the structure and diversity of intestinal flora in children with autism spectrum disorder (ASD). Methods The number of 20 children with ASD diagnosed in Maternal Child Health Hospital of Guangxi Autonomous Region and the number of 20 healthy children from January to June 2018 were selected as the subjects in the study.Total DNA was extracted from feces of two groups of children,and V4/V5 region of 16S rDNA was amplified for high throughput sequencing.The intestinal flora diversity of ASD children was analyzed by sequencing results. Results There were no significant differences in the coverage of sample libraries (Z=242.500,P=0.256),abundance index Chao1 (Z=250.000,P=0.181),ACE (Z=234.000,P=0.365),Shannon (Z=259.000,P=0.114) and Simpson (Z=146.000,P=0.145) indices between the two groups (P>0.05).At the gate level,there was no significant difference between the two groups in 8 species of bacteria (P>0.05),but at the generic level,there was significant difference between the two groups in 20 genera (P<0.05). Conclusions There are differences in intestinal flora between ASD children and healthy children.In clinic,the intestinal flora of ASD children can be accurately adjusted according to the characteristics of different bacteria genus to maintain the intestinal microecological balance and alleviate the gastrointestinal symptoms of ASD children.
Key words
intestinal flora /
autism spectrum disorder /
diversity /
children /
high throughput sequencing
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] 朱江,郭敏,杨亭,等.孤独症谱系障碍患儿胃肠道症状与行为表现关系研究[J].中华儿科杂志,2017,55(12):905-910.
[2] McElhanon BO,McCracken C,Karpen S,et al.Gastrointestinal symptoms in autism spectrum disorder:a meta-analysis[J].Pediatrics,2014,133(5):872-883.
[3] 刘杰,胡纯纯,徐秀.肠道微生物与神经发育及孤独症谱系障碍相关性的研究进展[J].中华实用儿科临床杂志,2017,32(19):1518-1520.
[4] 闻芳.肠道微生物与孤独症谱系障碍关系的研究进展[J].中国妇幼保健,2018,33(14):243-245.
[5] Regier DA,Kuhl EA,Kupfer DJ.The DSM-5:classification and criteria changes[J].World Psychiatry,2013,12(2):92-98.
[6] 邓琦蕾,申元英.肠道微生物群在脑-肠-微生物轴中作用机制的研究进展[J].实用医学杂志,2017,33(14):2404-2407.
[7] 刘杰,金锋,刘宇静,等.宏基因组学在自闭症儿童肠道菌群研究中的应用前景[J].中国医刊,2017,52(8):23-27.
[8] 傅锦坚,张玉,李红辉.孤独症与肠道菌群失衡的研究进展[J].中国儿童保健杂志,2017,25(3):255-257.
[9] 王宁.综合康复护理措施对孤独症儿童胃肠道问题的影响[J].齐鲁护理杂志,2015,21(11):43-44.
[10] 周自云.孤独症谱系障碍儿童饮食行为问题及肠道微生物的研究[D].合肥:安徽医科大学,2015.
[11] Finegold SM,Dowd SE,Gontcharova V,et al.Pyrosequencing study of fecal microflora of autistic and control children[J].Anaerobe,2010,16(4):444-453.
[12] Strati F,Cavalieri D,Albanese D,et al.New evidences on the altered gut microbiota in autism spectrum disorders[J].Microbiome,2017,5(1):24-24.
[13] Tomova A,Husarova V,Lakatosova S,et al.Gastrointestinal microbiota in children with autism in Slovakia[J].Physiol Behav,2015,138(0):179-187.
[14] 漆靖,蔡溢,肖剑英,等.抑郁症患者肠道菌群研究[J].中国微生态学杂志,2018,30(9):1057-1060.
[15] 孔维延,王瑜.肠道菌群与孤独症谱系障碍的研究进展[J].中国儿童保健杂志,2019,27(4):407-410
[16] Pifer R,Sperandio V.The interplay between the microbiota and enterohemorrhagic Escherichia coli[J].Microbiol Spectr,2015,2(5):421-436.
[17] Foley KA,MacFabe DF,Vaz A,et al.Sexually dimorphic effects of prenatal exposure to propionic acid and lipopolysaccharide on social behavior in neonatal,adolescent,and adult rats:implications for autism spectrum disorders[J].Int J Dev Neuro,2014,39:68-78.
[18] Borghi E,Borgo F,Severgnini M,et al.Rett syndrome:a focus on gut microbiota[J].Int J Mol,2017,18(2):344-344.
[19] 汪鸿,王小燕,吴梅荣,等.孤独症谱系障碍儿童外周血淀粉酶样前体蛋白及脑源性生长因子的特异性研究[J].中国儿童保健杂志,2018,26(10):95-98.
[20] 张梦想,王娟.孤独症谱系障碍与胃肠道疾病研究进展[J].生理科学进展,2016,47(4):300-304.
PDF(3512 KB)
