Study on differential expression of long non-coding RNA in peripheral blood of children with autism spectrum disorder

WEI Nian-jin; FANG Yu-dan; JIANG Lian; ZHENG Xiao-fei; HE Lin; MA Chen-huan; WANG Yu

doi:10.11852/zgetbjzz2019-0853

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Chinese Journal of Child Health Care ›› 2020, Vol. 28 ›› Issue (3) : 256-260. DOI: 10.11852/zgetbjzz2019-0853

Study on differential expression of long non-coding RNA in peripheral blood of children with autism spectrum disorder

WEI Nian-jin¹, FANG Yu-dan², JIANG Lian¹, ZHENG Xiao-fei¹, HE Lin¹, MA Chen-huan¹, WANG Yu¹

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Abstract

Objective To investigate the expression of LncRNA in peripheral blood of children with autism spectrum disorder (ASD),So as to provide theoretical basics for further study on early biomarkers of ASD. Methods Totally 40 ASD and normal control children were recuited from Shanghai Children′s Hospital from January to September 2018,and their peripheral blood samples were collected.The difference expression of whole-genome LncRNA between children with ASD and neurotypical controls was analyzed by the LncRNA Array v2.0 chip.The expression of LncRNA and mRNA related to neurodevelopment was quantitatively analyzed by qRT-PCR. Results A total of 3 655 LncRNA differential expression in ASD peripheral blood specimens were found according to the LncRNA Array v2.0 chip display,of which 2 238 LncRNA were upregulated,and 1 417 were downregulated.Quantitative analysis on 13 LncRNAs related to neurodevelopment by qRT-PCR showed that 10 LncRNAs were upregulated in ASD group,of which 4 LncRNAs were related to HOX gene and 3 LncRNAs were downregulated,1 LncRNAwas related to HOX gene.These results were in accordance with the study of array chip.The express of HOX gene in peripheral blood of ASD group was lower that of control group. Conclusions There are significant differences on LncRNA expression related to neurodevelopment in peripheral blood of ASD group.The differential expression of LncRNA related to HOX gene are potential biomarkers for early screen and diagnosis of ASD.

Key words

autism spectrum disorder / long noncoding RNA / homeobox gene

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WEI Nian-jin, FANG Yu-dan, JIANG Lian, ZHENG Xiao-fei, HE Lin, MA Chen-huan, WANG Yu. Study on differential expression of long non-coding RNA in peripheral blood of children with autism spectrum disorder[J]. Chinese Journal of Child Health Care. 2020, 28(3): 256-260 https://doi.org/10.11852/zgetbjzz2019-0853

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