Objective To investigate the relationship between inflammation response and the expression of miR-146a and miR-155 in peripheral blood mononuclear cells (PBMCs) of infants with respiratory syncytial virus (RSV) pneumonia. Methods Totally 84 children with RSV pneumonia admitted in the Third Clinical Medical College of Three Gorges University were enrolled in this study from January 2017 to January 2019,and were divided into mild group and severe group.Meanwhile,80 healthy children who took physical examination were selected as control group.Peripheral blood was collected and monocytes were isolated,real-time fluorescent quantitative PCR was used to test the levels of miR-146a and miR-155 in monocytes.The levels of serum tumor necrosis factor-α (TNF-α),interleukin-1β (IL-1β),interleukin-6 (IL-6) and interleukin-8 (IL-8) were detected by ELISA.Pearson method was used to analyze the correlation between the levels of inflammatory factors and the expression levels of miR-146a and miR-155 in the two groups. Results The level of miR-146a in PBMCs of severe group was significantly lower than that of mild group and control group (F＝31.907,P＜0.001),but miR-146a level was not significantly different between the mild group and the control group (P>0.05).The level of miR-155 in PBMCs of severe group was significantly higher than that of mild group and control group (F=143.127,P＜0.001),and it was significantly higher in mild group than control group (P＜0.001).The levels of serum TNF-α,IL-1β,IL-6 and IL-8 in children with RSV pneumonia were significantly higher than those in the control group (t=36.627,39.350,22.743,10.617,P＜0.001).MiR-146a level was negatively correlated with the expressions of TNF-α,IL-1β,IL-6 and IL-8 in children with RSV pneumonia (r=-0.473,-0.502,-0.396,-0.337,P＜0.05),while miR-155 level was positively related to the expressions of TNF-α,IL-1β,IL-6 and IL-8 (r=0.451,0.394,0.359,0.382,P＜0.05). Conclusion The expression of miR-146a in PBMCs of children with RSV pneumonia is low,while the expression of miR-155 is high,both of which are related to the severity of disease and inflammatory reaction in children.
Key words
respiratory syncytial virus /
pneumonia /
miR-146a /
mir-155 /
inflammatory factors /
infants
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