Objective To study the correlation between the activation of NOD-like receptor protein 3 (NLRP3) inflammasome and neurological damage in neonatal rat hyperbilirubinemia model.Methods A total of 36 3-days-old SPF-grade neonatal SD rats were randomly divided into control group,model group and VX group,with 12 rats in each group. The hyperbilirubinemia model of neonatal rats was established by intraperitoneal injection of 100 μg/g bilirubin in model group and VX group. The VX group was also given intraperitoneal injection of NLRP3 inflammasome inhibitor VX-765. The differences on bilirubin content in brain and serum,water content and ATP content in brain,Nissl staining and TUNEL staining,NSE and S100B contents in serum and the expression of NLRP3 inflammasome were analyzed among the three groups. Results The total bilirubin content,water content,TUNEL positive staining cell rate,NLRP3,ASC,cleaved-caspase-1,active-IL-1β,active-IL-18 expression levels in brain and serum total bilirubin,NSE,S100B contents in the model group were significantly higher than those in the control group (P<0.05). However,ATP content and the number of Nissl positive staining cells in the brain tissue were significantly lower than those in control group (P<0.05). The water content,TUNEL positive staining cell rate,cleaved-caspase-1,active-IL-1β,active-IL-18 expression level in brain and serum NSE,S100B contents in VX group were significantly lower than those in model group(P<0.05). ATP content and the number of Nissl positive staining cells in the brain tissue were significantly higher than those in model group (P<0.05). Conclusion NLRP3 inflammasome is involved in the process of nerve injury in neonatal rats with hyperbilirubinemia.
Key words
neonatal hyperbilirubinemia /
NOD-like receptor protein 3 /
inflammasome /
neurological damage
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