Objective To evaluate the expression level of cell death-inducingDNA fragmentation factor45-like effector C(CIDEC) in adipose tissue of obese children and its correlation with lipolysis and insulin resistance. Methods A total of 42 children who underwent surgery for non-inflammatory diseases such as hernia and cryptomchidism in the Second Affiliated Hospital of Xi′an Jiaotong University and Xi ′an Children′s Hospital were selected in this study from July 2014 to September 2016,and were divided into obesity group and control group.The body mass index (BMI),waist circumference,blood pressure,blood lipids,fasting blood glucose,serum free fatty acid (NEFA) and insulin resistance index (HOMA-IR) of children were measured.Meanwhile,western blot and RT-PCR were applied to detect the expression level of CIDEC,tumor necrosis factor-α(TNF-α),peroxisome proliferators-activated receptors (PPARγ).And the associations among TNF-α,NEFA,HOMA-IR and CIDEC were studied by Pearson correlation analysis. Results 1) Indexes in obese children,including BMI,waist circumference,systolic blood pressure (SBP),diastolic blood pressure (DBP),HOMA-IR and NEFA,were significantly higher than those in normal controls(P＜0.05),while HDL-C was relatively lower in obese group.2) The expression level of CIDEC mRNA and protein in adipose tissue of obese group decreased (P<0.01),while the expression of TNF-α mRNA increased (P<0.01).3) Correlation analysis showed that the expression level of CIDEC mRNA in adipose tissue was negatively correlated with the levels of TNF-α mRNA,HOMA-IR,NEFA (r=-0.583,-0.560,-0.606,P＜0.05). Conclusions TNF-α causes adipocyte lipolysis,thereby leading to insulin resistance,possibly resulting from down-regulating the expression of CIDEC.
Key words
obesity /
children /
cell death-inducing DNA fragmentation factor 45-like effector C /
insulin resistance /
tumor necrosis factor-α
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] 季成叶.中国学生超重肥胖BMI筛查标准的应用[J].中国学校卫生,2004,25(1):125-128.
[2] Samuel VT,Shulman GI.Mechanisms for insulin resistance:common threads and missinglinks[J].Cell,2012,148(5):852-871.
[3] Ouchi N,Parker JL,Lugus JJ,et al.Adipokines in inflammation and metabolic disease[J].Nat Rev Immunol,2011,11(2):85-97.
[4] Lumeng CN,Bodzin JL,Saltiel AR.Obesity induces a phenotypic switch in adipose tissue macrophage polarization[J].J Clin Invest,2007,117(1):175.
[5] Park YS,Uddin MJ,Piao L,et al.Novel role of endogenous catalase in macrophage polarization in adipose tissue.[J].Mediators Inflamm,2016,2016:8675905
[6] Fain JN,Bahouth SW,Madan AK.TNFαlpha release by the nonfat cells of human adipose tissue[J].Int J Obes Relat Metab Disord,2004,28(4):616-622.
[7] Zhou W,Yang P,Liu L,et al.Transmembrane tumor necrosis factor-alpha sensitizes adipocytes to insulin[J].Mol Cell Endocrinol,2015,406:78-86.
[8] Palacios-Ortega S,Varela-Guruceaga M,Algarabel M,et al.Effect of TNF-alpha on caveolin-1 expression and insulin signaling during adipocyte differentiation and in mature adipocytes[J].Cell Physiol Biochem,2015,36(4):1499-1516.
[9] Dahlman I,Forsgren M,Sjögren A,et al.Downregulation of electron transport chain genes in visceral adipose tissue in type 2 diabetes independent of obesity and possibly involving tumor necrosis factor-α[J].Diabetes,2006,55(6):1792-1799.
[10] Jambunathan S,Yin J,Khan W,et al.FSP27 promotes lipid droplet clustering and then fusion to regulate triglyceride accumulation[J].PloS One,2011,6(12):e28614.
[11] Gong J,Sun Z,Wu L,et al.Fsp27 promotes lipid droplet growth by lipid exchange and transfer at lipid droplet contactsites[J].J Cell Biol,2011,195(6):953-963.
[12] Nian Z,Sun Z,Yu L,et al.Fat-specific protein 27 undergoes ubiquitin-dependent degradation regulated by triacylglycerol synthesis and lipid droplet formation[J].J Biol Chem,2010,285(13):9604-9615.
[13] 李红强.CIDEC在脂滴形成中的功能[D].武汉:华中农业大学,2012.
[14] Yang R,Guan MJ,Zhao N,et al.Roles of extrahepatic lipolysis and the disturbance of hepatic fatty acid metabolism in TNF-α -induced hepatic steatosis[J].Toxicology,2019,411:172-180.
PDF(936 KB)
