Objective To discuss the effectiveness of lipopolysaccharide(LPS) intrauterine injection for establishing a premature rat model and the changes of learning and memory ability of the preterm birth pups. Methods A preterm birth model induced by intrauterine immflammation of SD pregnant rats was established.Thirty pregnant rats were randomly divided into experimental group and control group,with 15 rats in each group.All rats of the two groups were intrauterinely injected with 100 μl 25%LPS solution or 100 μl physiological saline on the 17th day of pregnancy,then the pregnancy outcome was observed.Navigation experiment and space exploration experiment of morris water maze were conducted in the first month after birth to test the changes of ability on the spatial learning and memory of the offspring. Results The preterm birth rate and stillbirth rate of the experimental group were significantly higher than those of control group(preterm birth:100% vs.6.7%,χ2=11.46,P<0.05; stillbirth:2.9% vs.0.9%,χ2=8.82,P<0.05).The average number of per litter(t=4.83,P=0.02),the average weight of offspring(t=3.75,P=0.03) and the survival rate of offspring in the first month after birth(χ2=3.68,P=0.03) in experimental group were significantly lower than those in control group.In the Morris water maze task,the experimental group adopted more non-effective strategies,while the control group adopted more effective strategies,and the difference was significant(χ2=4.27,P=0.02).The average incubation period of one-month-old rats in the experimental group was significantly longer than that in the control group(t=6.92,P=0.001).While the station stay time of one-month-old pups of experimental group was significantly shorter than that of the control group in space exploration experiment(t=5.28,P=0.005). Conclusions LPS intrauterine injection is a modeling method with high feasibility and success rate,which will result in impaired learning and memory ability of early litter rats.So it can be used in the related studies on premature labor caused by intrauterine inflammation.
Key words
preterm /
intrauterine /
offspring /
learning and memory /
lipopolysaccharide
{{custom_sec.title}}
{{custom_sec.title}}
{{custom_sec.content}}
References
[1] Cordeiro CN,Tsimis M,Burd I. Infections and brain development[J]. Obstet Gynecol Surv,2015,70(10):644-55.
[2] 宗璐,郭玉琳,苟文丽,等. 孕期补锌对胎儿生长受限仔鼠学习记忆能力改变的研究[J].中国儿童保健杂志,2015,23(10):1047-1050.
[3] Roberto R,Jean-Charles G,Adi LT,et al. Evidence of perturbations of the cytokine network in preterm labor[J]. Am J Obstet Gynecol,2015,213(6):836.
[4] Blencowe H,Lawn JE,Vazquez T,et al.Preterm-associated visual impairment and estimates ofretinopathy of prematurity at regional and global levels for 2010[J]. Pediatr Res,2013,74(Suppl 1):35-49.
[5]Jun L,Jason MR,Manoj KM,et al. Maternal dendrimer-based therapy for inflammation-induced preterm birth and perinatal brain injury[J].Sci Rep,2017,7:6106
[6]Valeria GF,Roberto R,Derek M,et al. Inflammation-induced adverse pregnancy and neonatal outcomes can be improved by the immunomodulatory peptide exendin-4[J].Front Immunol,2018,9:1291.
[7] Pujol Lopez Y,Steinbusch HW,Rutten B,et al. Effects of subcutaneous LPS injection on gestational length and intrauterine and neonatalmortality in mice[J]. Neuroimmunomodulation,2015,22(4):274-278.
[8] Lauren LJ,Shenandoah R. Preclinical models of encephalopathy of prematurity[J].Dev Neurosci,2015,37:277-288.
[9] David F,Covadonga M,Alberto RQ,et al. Oxidative stress and inflammation in early onset first episode psychosis:a systematic review and Meta-analysis[J].Int J Neuropsychopharmacol,2017,20(6):435-444.
[10] Henrik H,Carina M,Donna MF,et al.The role of inflammation in perinatal brain injury[J].Nat Rev Neurol,2015,11(4):192-208.
[11] 石计朋,栗延伟,宋亚辉,等. ω-3多不饱和脂肪酸对脂多糖所致新生大鼠脑损伤Toll-NF-κB信号通路调节作用[J].中国儿童保健杂志,2018,26(2):158-162.
PDF(1377 KB)
