Objective To investigate the association of inflammatory cytokines including interleukin-6 (IL-6), IL-6R, IL-1β and IL-1RN gene polymorphism with severity of hand foot and mouth disease (HFMD) induced by enterovirus 71 (EV71), in order to provide evidence for early recognition and assessment of severe HFMD.Methods A total of 180 EV71 HFMD cases including 108 severe cases and 72 mild cases from the Second Affiliated Hospital of Xi′an Jiaotong University and Xi′an Children′s Hospital were randomly enrolled in this study. All participants were genotyped for the most common IL-6, IL-6R, IL-1β and IL-1RN single-nucleotide polymorphisms (SNPs) using the SNPscan multiple SNP typing method. The gene type of rs1800796, rs2069837 of IL-6, rs2228145, rs4845617 of IL-6R,rs16944, rs1143627 of IL-1β and rs315952, rs9005 of IL-1R among 180 cases were detected by SNPscan kit.Results The severity risk was significantly increased in IL-6 rs1800796 allele G(OR=1.312, 95% CI:1.041~1.653)and IL6-R rs2228145 allele C (OR=1.330, 95% CI:1.064～1.663), while it was reduced in IL-1β rs1143627 allele G (OR=0.790,95% CI:0.635~0.981). No association of gene polymorphism with severity of EV71 HFMD was observed in IL-6 rs2069837, IL-6R rs4845617, IL-1β rs16944, IL-1RN rs315952 and IL-1RN rs9005.Conclusion The gene polymorphism of IL-6 rs1800796, IL-6R rs2228145 and IL-1β rs1143627 were associated with severity of EV71 HFMD.
Key words
enterovirus 71 /
interleukin-6 /
interleukin-1β /
polymorphism /
severity
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