Effects of ethyl pyruvate on expression of aquaporin-4 and ultrastructure changes in neonatal rats with brain damage caused by intrauterine infection

SONG Fu-xiang; KONG Xiang-ying; PANG Wei; TAN Li-ping; GUO Jin

doi:10.11852/zgetbjzz2018-26-11-09

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Chinese Journal of Child Health Care ›› 2018, Vol. 26 ›› Issue (11) : 1194-1198. DOI: 10.11852/zgetbjzz2018-26-11-09

Effects of ethyl pyruvate on expression of aquaporin-4 and ultrastructure changes in neonatal rats with brain damage caused by intrauterine infection

SONG Fu-xiang, KONG Xiang-ying, PANG Wei, TAN Li-ping, GUO Jin

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Abstract

Objective To study the effect of ethyl pyruvate (EP) in the expression of aquaporin-4(AQP4) and ultrastructure changes in neonatal rats with brain damage caused by intrauterine infection.Methods A total of 36 pregnant Wistar rats were enrolled in this study, and were divided into lipopolysaccharide (LPS) group, EP group and normal saline(NS) group, with 12 rats in each group. LPS group was consecutively injected with LPS (380 μg/(kg·d) intraperitoneally, EP group were consecutively injected with lipopolysaccharide intraperitoneally, and EP was administrated intraperitoneally at a dose of 40 mg/kg immediately. NS group was injected with normal saline intraperitoneally as control group. After the delivery of pups, thirty-six pups were selected. Placenta of rats were taken for hematoxylline-eosin (HE) staining to confirm its intrauterine infection in 12 hours, 24 hours,48 hours after birth. Aquaporin-4 expression in brain tissue was studied through immunohistochemical analysis. Ultrastructure changes in damaged brain and control group were studied electron-microscopically.Results Obvious pathological changes were observed in the placenta of LPS group. The expression level of AQP4 was lower in EP group and NS group in 24 hours and 48 hours after birth than that in LPS group (P<0.05). LPS group nerve cells ultrastructure showed serious injury.Conclusion Ethyl pyruvate has neuro-protective effect on LPS-induced brain injury, and may be associated with the expression level of AQP4.

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ethyl pyruvate / intrauterine infection / brain injury / AQP4 / ultrastructure

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SONG Fu-xiang, KONG Xiang-ying, PANG Wei, TAN Li-ping, GUO Jin. Effects of ethyl pyruvate on expression of aquaporin-4 and ultrastructure changes in neonatal rats with brain damage caused by intrauterine infection[J]. Chinese Journal of Child Health Care. 2018, 26(11): 1194-1198 https://doi.org/10.11852/zgetbjzz2018-26-11-09

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