Effect of antibiotics use on the weight and mortality in newborn mice

ZHANG Xin; LI Shao-wen; GAO Xiao-cui; CHEN Jia-yan

doi:10.11852/zgetbjzz2018-26-10-12

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Chinese Journal of Child Health Care ›› 2018, Vol. 26 ›› Issue (10) : 1088-1091. DOI: 10.11852/zgetbjzz2018-26-10-12

Effect of antibiotics use on the weight and mortality in newborn mice

ZHANG Xin¹, LI Shao-wen², GAO Xiao-cui¹, CHEN Jia-yan¹

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Abstract

Objective To explore the effect of antibiotics use in early life on weight and mortality of newborn mice . Method A total of 40 female wild-type C57BL/6 mice aged 6～8 weeks and 20 male wild-type C57BL/6 mice aged 6~8 weeks were randomly divided into control group and antibiotic treatment group, with 20 mice in each group. Two female mice in the control and antibiotic groups were paired with one male mouse in a cage for 12 weeks. The mice in the control group were given normal feed and water, while the mice in the antibiotic group were given both normal feed and antibiotic cocktail. The weight of newborn mice was measured each week, fecal bacterial DNA was tested, and mortality was counted. Results The overall mortality rate of newborn mice in the antibiotic group was significantly higher than that in the control group (P=0.034, 0.027) within 1 week and 1～2 weeks after birth. There was no significantly gender difference in mortality between 1 week and 1～2 weeks after birth (P＞0.05). The weight of newborn mice in the control group and the antibiotic group at 1 week after birth was(3.870±0.079)g and(4.050±0.050)g, respectively, with no significant difference between the two groups (t=1.926, P=0.070). At 2 weeks, the weight was (6.230±0.142)g and (6.770±0.141)g, respectively. The weight of the antibiotic newborn mice was significantly higher than that of the control group at 2 weeks after birth (t=2.699, P=0.015). Moreover, the fecal DNA concentration of newborn mice in the antibiotic group was significantly lower than that of the control group (P=0.007) at 1 week and 2 weeks. Conclusions The use of antibiotics in early life in newborn mice significantly reduces the number of intestinal bacteria, increases weight and mortality, and the mechanism remains to be further studied.

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antibiotics / cocktail / weight / mortality / newborn mice

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ZHANG Xin, LI Shao-wen, GAO Xiao-cui, CHEN Jia-yan. Effect of antibiotics use on the weight and mortality in newborn mice[J]. Chinese Journal of Child Health Care. 2018, 26(10): 1088-1091 https://doi.org/10.11852/zgetbjzz2018-26-10-12

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