Objective To explore the impact of the assisted reproductive technology (ART) on the offsprings′ general movement assessment (GMs) by applying propensity score matching . Method Infants who had received at least one time of GMs were collected in Minhang district from July 2015 to October 2017.Infants were divided into two groups of ART conceived and natural conceived.The first time of GMs results of the two groups during the two stages of “premature and twist” and “restless movement ” were matched by applying the one-to-one propensity score matching (PSM),setting the matching tolerance 0.000 03 and 0.000 15 respectively,with the gender,fetus number,birth weight,gestational age,the history of rescue,jaundice,brain and other disease,parental age,culture,occupation,maternal fetal miscarriage,medication,diabetes,or hypertension the history of other diseases as covariates.The differences between the two groups were compared. Results Before matched, the rate of abnormal result of two GMs stages in ART conceived group was significantly higher than that in natural conceived group(χ²=77.74,15.38,both P＜0.01).After matched,there were 164 pairs of data in the stage of premature and twist,and the rate of abnormal result of ART conceived group was significantly higher than that of natural conceived group (32.3% vs.18.9%,χ²=8.30,P=0.008).However,there was no significant difference between the two groups of 247 pairs of data in the stage of restless exercise (P>0.05). Conclusions ART may increase the risk of abnormal result in the stage of “premature and twist”,so further research is needed.