Preliminary study on the difference analysis of urine metabolitesat birth in preterm infants with retinopathy of prematurity

CAI Yao; LEI Hui; HAO Hu; YANG Qiu-ping; LI Fei; GU Xia; LI Si-tao

doi:10.11852/zgetbjzz2018-26-09-16

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Chinese Journal of Child Health Care ›› 2018, Vol. 26 ›› Issue (9) : 991-994. DOI: 10.11852/zgetbjzz2018-26-09-16

Preliminary study on the difference analysis of urine metabolitesat birth in preterm infants with retinopathy of prematurity

CAI Yao¹, LEI Hui², HAO Hu¹, YANG Qiu-ping¹, LI Fei¹, GU Xia¹, LI Si-tao¹

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Abstract

Objective To analyze the composition and differences of important metabolites/products in premature infants without retinopathy of prematurity(non-ROP) and premature infants with retinopathy of prematurity(ROP), in order to find biomarkers closely related to the development of ROP. Methods Totally 21 premature infants with gestational age of 27~34 weeks and diagnosed with ROP in the Sixth Affiliated Hospital of Sun Yat-Sen University were enrolled as experimental group, and 21 premature infants without ROP were selected as control group from January 2013 to December 2016. Urine samples of the participants were collected and the Gas Chromatography-Mass Spectometry (GC/MS)was used to generate the urine metabolites results.Data collected were subjected to the metabolomics analysis by using the orthogonal partial least squares discriminant analysis to search for the considerable biomarkers. Results The urine metabolites from the ROP group and the non-ROP group displayed a clearly differentiated metabolic profiles.Ten metabolites were identified which possessed the biggest weight discriminating the ROP group from the non-ROP group in the pattern of recognizing process combined with the metabolites score plot with variable importance plot. ROP group had lower levels of methionine,3-amino-isobutyric acid and hippuric acid,while the level of 3-hydroxy-3-methylglutaric acid were significantly higher in the ROP group (all P＜0.05). Conclusion Urine metabolites including methionine,3-amino-isobutyric acid, hippuric acid and 3-hydroxy-3-methylglutaric acid can be selected as the biological markers for the early diagnosis of ROP.

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gas chromatography-mass spectrometry / retinopathy of prematurity / premature infant / urine metabolites

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CAI Yao, LEI Hui, HAO Hu, YANG Qiu-ping, LI Fei, GU Xia, LI Si-tao. Preliminary study on the difference analysis of urine metabolitesat birth in preterm infants with retinopathy of prematurity[J]. Chinese Journal of Child Health Care. 2018, 26(9): 991-994 https://doi.org/10.11852/zgetbjzz2018-26-09-16

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