Objective To monitor virus pollution of environmental samples of 2 kindergartens where norovirus outbreaks occurred in Jingmen city,in order to provide evidence for the early disinfection and health management in the early stage of norovirus epidemic. Method According to real time PCR (RT-PCR) method published by the Chinese Center for Disease Control and Prevention (2015 Edition), norovirus nucleic acid in biological samples of kindergarten patients, food, water, tableware, sanitary products, toys, mop, door curtain, water stopcocks and other environmental samples were collected and tested. Results The positive rate of norovirus were 63.64% (7/11) in stockings with soap bags;50.00%(5/10)in mop; 44.44%(4/9)in cups;40.00%(2/5)in door curtain; 22.22%(2/9)in toys; 16.67%(1/6)in water stopcocks;11.11% (1/9) in tableware; 8.33%(1/12)in food;0.00% (0/6) in doorknob and drinking water. Conclusions Stockings with soap bags,mop, cups, door curtain and other environmental samples are prone to be contaminated by norovirus when norovirus epidemic occurs in kindergartens.It is suggested to focus on these areas and make reasonable disinfection measures,in order to prevent further spread and outbreak of norovirus.