Objective To discuss the effect of saccharomyces boulardii and early intervention on NF-kB expression in brain tissue, as well as learning and memory in neonatal rats with hyperbilirubinemia. Methods A total of 88 SD rats aged 7 days were randomly divided into the control group (NS group) and experiment group (T1,T2 and T3 group), with 22 rats in each group. The experiment group was intraperitoneally injected with 50 μg/g bilirubin at 7 days and 10 days respectively, and the control group was injected intraperitoneally with equal volume of normal saline. After the last injection of 12 h, 6 neonatal rats were randomly selected in each group, and the bilirubin contents in the brain and serum were measured. Meantime, group T1 was treated with saccharomyces boulardii, and group T2 was treated with saccharomyces boulardii and early intervention, group T3 was untreated till 28 days old. HE was used to observe the pathological changes of brain tissue, the NF-kB positive cells were observed by immunohistochemical staining, and morris water was used to evaluate the latency and the times crossing target quadrants. Results The bilirubin contents in the brain and serum of NS group with 10-day-old rats were significantly lower than those in the experiment group (P<0.01), but there was no significant difference among the experiment groups (P>0.05). The bilirubin contents in the brain and serum of NS group with 28-day-old rats were significantly lower than those in group T1, T2 and T3, and the bilirubin contents in the brain and serum in group T1 were significantly lower than those in group T1 and T3 (P<0.05). HE results showed that the neurons of NS group had complete structure, but the number of neurons in group T1,T2 and T3 were decreased and with bilirubin deposits. The number of NF-kB positive cells in NS group were significantly less than those in group T1,T2 and T3 (P<0.01), and were also less in group T2 than that in group T1 and T3 (P<0.05). The latency and the times crossing target quadrants in NS group were significantly better than those in group T1、T2 and T3 (P<0.05), and were also better in group T2 than that in group T1 and T3 (P<0.05). Conclusion Saccharomyces boulardii and early intervention can effectively reduce expression of NF-kB in brain tissue and improve learning and memory in neonatal rats with hyperbilirubinemia.
Key words
hyperbilirubinemia /
saccharomyces boulardii /
early intervention /
NF-kB /
learning and memory /
rats
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