Objective To analyze the epidemjological characteristics of bacterial and viral infectious diarrhea of kindergarten children in Pudong New Area. Methods Relying on 6 hospitals, the stool samples and epidemiological data of diarrhea cases in kindergarten children were collected according to the sampling distance of 1∶5~1∶20, and the stool samples were used to test for bacteria and viruses, at the meantime the epidemiological data was for epidemiology analysis. Results Among 381 stool samples detected, 232 (60.89%)were bacterial and viral pathogen positive, the positive rate in private kindergartens was higher than that in public kindergartens(χ²=6.53,P＜0.05).The bacterium positive rate was 10.24%, and the main pathogen was pathogenic Escherichia coli; The virus positive rate was 33.33%, and the main pathogens were norovirus and rotavirus. The cross infection positive rate was 17.59%, mainly were combined with 2 viruses. In summer, the bacterium positive rate was higher than virus, but the virus positive rate was higher in winter and spring(χ²=34.00,P＜0.05). More cases had vomiting in virus positive group (42.06%) than in bacterium positive group (17.95%). Conclusions The bacterial and viral pathogen spectrum of infectious diarrhea is related to season obviously in Pudong New Area kindergarten children, and children in private kindergarten are risk groups. It is necessary to carry out prevention and control measures for different seasons and groups.